Ovalbumin-sensitized skin from CysLT2 receptor knock-out mice on the BALB/c background fails to develop significant skin thickening or collagen deposition. This strain may be useful for studying atopic dermatitis.
Yoshihide Kanaoka, Brigham and Women's Hospital
Cysltr2 (cysteinyl leukotriene receptor 2) encodes a member of the superfamily of G protein-coupled receptors and functions as a receptor for the cysteinyl leukotrienes. Cysteinyl leukotrienes are lipid mediators associated with allergic and asthmatic inflammatory responses. The CysLT2 receptor knock-out mice contain a neomycin cassette inserted into exon 6 of Cysltr2. Homozygous null mice are viable and fertile. Tape-stripped ovalbumin-sensitized skin from BALB/c congenic homozygous mice develops reduced epidermal and dermal thickening and collagen deposition as compared to treated skin from controls. This strain may be useful for studying atopic dermatitis.
The Cysltr2tm1Ykn allele is also available on the C57BL/6 background (Stock No. 031624), however, atopic dermatitis has not been tested on this background.
The targeting vector was designed to use a neomycin resistance gene to disrupt exon 6 of the Cysltr2 gene. The insertion targets 264 amino acids in the N-terminal and interrupts 85% of the coding region. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6NCrl females and offspring were backcrossed to BALB/c mice for at least 10 generations. Northern blot analysis of lung, spleen and small intestine does not detect RNA transcript. Upon arrival, mice were bred to BALB/cJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Yoshihide Kanaoka|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Yoshihide Kanaoka; Cysltr2tm1Ykn|
|Gene Symbol and Name||Cysltr2, cysteinyl leukotriene receptor 2|
|Gene Synonym(s)||CYSLT2; CYSLT2R; HG57; RIKEN cDNA 2300001H05 gene; HPN321; KPG_011; 2300001H05Rik; 2300001H05Rik; PSEC0146; RSBPT32; GPCR21; hGPCR21; CysLT2; Cyslt2|
|Strain of Origin||C57BL/6|
|Molecular Note||The insertion of a neo into exon 6 interrupted 85% of the coding sequence. Northern blot showed a lack of transcript in mutant lung, spleen and small intestine.|
When maintaining a live colony, these mice are bred by homozygote sibling matings.
When using the Cysltr2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #031718 in your Materials and Methods section.
|Heterozygous for Cysltr2<tm1Ykn>|
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