B6.129P2(Cg)-Il4/Il13^tm1.1Lky/J

Stock number: 031366
Product name: B6 4-13 , 4-13floxed
Research areas: Neurobiology Research, Research Tools

Description

Il4/Il13^flox mutant mice, backcrossed to C57BL/6J for more than ten generations, harbor loxP-flanked sequences that span the adjacent genes Il4 and Il13, enabling the conditional disruption of both genes simultaneously via Cre-induced recombination. There are no overt phenotypic differences between this C57BL/6J backcrossed line and the original BALB/c background line (see Stock No. 015859).

Detailed description

These Il4/Il13^flox mutant mice possess a loxP site upstream of exon 3 of the interleukin 13 (Il13) gene and a second loxP site downstream of exon 4 of the interleukin 4 (Il4) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have nucleotides between exons 3 of Il13 and exon 4 of Il4 deleted in the cre-expressing tissue. This strain may be useful for studying immune function in mice lacking Il4 and Il13.

There are no overt phenotypic differences between this C57BL/6J backcrossed line and the original BALB/c background line (see Stock No. 015859).

Development

A targeting vector was designed to insert a loxP-flanked blasticidin (bsd) resistance cassette upstream of exon 3 of the interleukin 13 (Il13) gene, and a frt-flanked neomycin resistance cassette followed by a single loxP site downstream of exon 4 of the interleukin 4 (Il4) gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pMC-CreN expression plasmid to delete the bsd cassette. Resulting ES cells contained multiple gene rearrangements; intact floxed exons, intact floxed bsd cassette, or excision of both the exons and the bsd cassette. Correctly targeted ES cells, containing only the floxed exons, were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6 females. Mice heterozygous for the Il4/Il13^flox allele were bred to mice containing Gt(ROSA)26Sor^tm1(FLP1)Dym to delete the neo cassette. This line was backcrossed to BALB/c mice for at least ten generations prior to being backcrossed to C57BL/6J for more than ten generations by the donating laboratory.

Allele

Symbol: Il4/Il13^tm1.1Lky
Name: targeted mutation 1.1, Richard M Locksley
MGI accession ID: MGI:4431367
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: 4-13^F; Il4-IL13^fl
Marker symbol: Il4
Marker name: interleukin 4
Marker synonyms: BCGF1; BCGF-1; BSF1; BSF-1; IL-4; Il4e12
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: A single targeting event was used to insert a floxed Bsd resistance cassette between exon 2 and 3 of Il13 and an frt flanked neo cassette with a 3' loxP site downstream of exon 4 of Il4 . Flp and cre mediated recombination removed the selection cassettes leaving the region between exon 3 of Il13 and exon 4 of Il4 floxed.

Allele

Symbol: Il4/Il13^tm1.1Lky
Name: targeted mutation 1.1, Richard M Locksley
MGI accession ID: MGI:4431368
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: 4-13^F
Marker symbol: Il13
Marker name: interleukin 13
Marker synonyms: Il-13; P600
Chromosome: 11
Strain of origin: 129P2/OlaHsd
Molecular note: A single targeting event was used to insert a floxed Bsd resistance cassette between exon 2 and 3 of Il13 and an frt flanked neo cassette with a 3' loxP site downstream of exon 4 of Il4 . Flp and cre mediated recombination removed the selection cassettes leaving the region between exon 3 of Il13 and exon 4 of Il4 floxed.