The Villin-rtTA*M2 transgene contains the mouse villin 1 (<i>Vil1</i>) promoter directing expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein. Founder line 3 (V3) homozygous mice are viable and fertile. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed in intestinal villi (founder line 3). 

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Villin-rtTA*M2 line 3 (V3) transgenic mice have the mouse villin 1 regulatory elements driving expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein restricted to intestinal villi. This strain provides a Tet-On tool that allows the inducible expression of genes in the intestinal villi.

A minimum of two mice must be ordered. Additional mice will be charged on a per-mouse basis. Click the “Details” button to learn more.

Our minimum guarantee for cryorecovery orders is 2 carriers. To ensure that your colony can be rapidly expanded upon arrival, additional carriers may be added to your order.