B6.129S-Jag1^tm2Grid/SjJ

Stock number: 031272
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 4, which encodes the DSL (Delta-Serrate-Lag2) domain, of the Jag1 gene. This strain may be useful for generating conditional mutations for studies of Notch signaling during development.

Detailed description

These mice possess loxP sites on either side of exon 4, which encodes the DSL (Delta-Serrate-Lag2) domain, of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissue(s). The exon 4-deleted allele produces a nonfunctional JAG1 protein.

When bred to a strain with Cre recombinase expression during development in the telencephalon and discrete head structures, such as the otocyst (see Stock No. 006084 for example), this mutant mouse strain may be useful in studies of developmental inner ear defects.

When bred to a strain with Cre recombinase expression in endothelial cells during embryogenesis and adulthood (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of congenital heart defects and ductus arteriosus development.

When bred to mice carrying Tg(Wnt1-cre)11Rth (Stock No. 009107), Cre recombinase expression in the midbrain and developing neural tube results in a craniofacial phenotype characteristic of Alagille Syndrome.

Development

JAX Stock No. 010618 was backcrossed to C57BL/6J for 10 generations by the donating laboratory to create this congenic strain.

A FRT site flanked targeting vector containing a PGK-neo cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were crossed to C57BL/6J females. Heterozygotes were intercrossed to generate homozygotes. The mice were then crossed to 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J (STOCK no. 003946) to remove the PGK-neo selection cassette. These mice no longer carry the Gt(ROSA)26Sor^tm1(FLP1)Dym allele.

Allele

Symbol: Jag1^tm2Grid
Name: targeted mutation 2, Tom Gridley
MGI accession ID: MGI:3692444
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Jag1^flox
Marker symbol: Jag1
Marker name: jagged 1
Marker synonyms: ABE2; AGS; AGS1; AHD; AWS; CD339; CMT2HH; DCHE; Gsfabe2; Headturner; HJ1; Htu; JAGL1; Ozz; Serrate-1
Chromosome: 2
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exon 4 was flanked by loxP sites. An FRT-flanked neo was removed from the locus.