This CRISPR-generated knock-out mutant of the troponin C, cardiac/slow skeletal (Tnnc1) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Tnnc1 encodes a component of Troponin, a regulatory protein of striated muscle contraction. Tnnc1 abolishes the inhibitory action of Troponin on actin filaments.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using CRISPR technology. The targeted gene, troponin C, cardiac/slow skeletal (Tnnc1), encodes a component of Troponin, a regulatory protein of striated muscle contraction. Tnnc1 abolishes the inhibitory action of Troponin on actin filaments. The alteration resulted in the deletion of 222 bp, which should result in the deletion of exon 4, amino acid change after residue 67, and early termination 8 amino acids later. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (CCCCTCCTCCCCACACTATA, AATGCTAACTGTTCTCTCTC and TGGCCCAAGCAGAGAGGAGA), designed to delete 222 bp in exon 4 of the troponin C, cardiac/slow skeletal (Tnnc1) gene, and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by PCR and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Tnnc1, troponin C, cardiac/slow skeletal|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 3 guide sequences CCCCTCCTCCCCACACTATA, AATGCTAACTGTTCTCTCTC and TGGCCCAAGCAGAGAGGAGA, which resulted in a 222 bp deletion beginning at Chromosome 14 positive strand position 31,210,481 bp and ending after 31,210,702 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001063990(exon 4) and 107 bp of flanking intronic sequence including the splice acceptor and donor. In addition there is a 6 bp intronic deletion (GAGAAG) 18 bp after the 222 bp deletion that will not alter the results of the exon deletion. This mutation is predicted to cause a change of amino acid sequence after residue 67 and early truncation 8 amino acids later.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. As mice are characterized, data related to viability and fertility will be provided on the International Mouse Phenotyping Consortium website.
When using the C57BL/6NJ-Tnnc1em1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #42360 in your Materials and Methods section.