This CRISPR-generated knock-out mutant of the Sin3-associated polypeptide 18 (Sap18) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Sap18 encodes a component of the histone deacetylase complex which mediates transcriptional repression.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using CRISPR technology. The targeted gene, Sin3-associated polypeptide 18 (Sap18), encodes a component of the histone deacetylase complex which mediates transcriptional repression. The alteration resulted in the deletion of 432 bp, which should result in the deletion of exon 2, amino acid change after residue 43, and early termination 9 amino acids later. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (TGCGGGTTGCGGGCGGCTGC, TTTAATATCTTGGTGCTGCGand GCGGTAGGATAAGATGGTAG), designed to delete 432 bp in exon 2 of the Sin3-associated polypeptide 18 (Sap18) gene, and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by PCR and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Sap18, Sin3-associated polypeptide 18|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 3 guide sequences TGCGGGTTGCGGGCGGCTGC, TTTAATATCTTGGTGCTGCGand GCGGTAGGATAAGATGGTAG, which resulted in a 432 bp deletion beginning at Chromosome 14 positive strand position 57,798,370 bp and ending after 57,798,801 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000122492 (exon 2) and 322 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 43 and early truncation 9 amino acids later.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. As mice are characterized, data related to viability and fertility will be provided on the International Mouse Phenotyping Consortium website.
When using the C57BL/6NJ-Sap18em1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #42359 in your Materials and Methods section.