C.FVB-Tg(Cspg4-ZsYellow1)1Rkl/J

Stock number: 031158
Product name: NG2-YFP, ZsYellow1
Research areas: Research Tools

Description

The NG2-YFP transgenic strain is an enhanced yellow fluorescent (ZsYellow1, from Zoanthus sp.) reporter for Cspg4 expressing pericytes, and has applications in studies related to the role of Cspg4 expressing pericytes in cancer and fibrosis.

Detailed description

These NG2-YFP transgenic mice express ZsYellow1, an enhanced yellow fluorescent protein from Zoanthus sp., under control of the mouse Cspg4 (chondroitin sulfate proteoglycan 4) gene promoter. This strain is a fluorescent reporter for Cspg4 expressing pericytes. In normal kidney ZsYellow1 fluorescence is largely restricted to large and small vascular structures including the glomeruli. In fibrotic kidney, NG2-YFP expression is detected in renal interstitial cells and vascular structures. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (October 2017).

Development

The NG2-YFP transgenic construct containing ZsYellow1 (from pZsYellow-N1 (Clontech), a human codon-optimized yellow fluorescent protein from Zoanthus sp., under the control of the approximately 5kb mouse Cspg4 gene promoter and part of exon 1 of the Cspg4 gene (with a mutant ATG start codon) was injected into fertilized FVB/N mouse eggs. Founder line 1 was subsequently established. The mice were then backcrossed to BALB/cAnNCrl for more than 10 generations.

Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize BALB/cByJ oocytes (Stock No. 001026).

Allele

Symbol: Tg(Cspg4-ZsYellow1)1Rkl
Name: transgene insertion 1, Raghu Kalluri
MGI accession ID: MGI:6100934
Generation method: Transgenic
Attributes: Reporter
Synonyms: NG2-YFP
Marker symbol: Tg(Cspg4-ZsYellow1)1Rkl
Marker name: transgene insertion 1, Raghu Kalluri
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: ZsYellow1,ZsYellow1 yellow fluorescent protein, Zoanthus,zoanthid
Site of expression: In normal kidney yellow fluorescence (ZsYellow1) is largely restricted to large and small vascular structures including the glomeruli. In fibrotic kidney, NG2-YFP expression is detected in renal interstitial cells and vascular structures.
Molecular note: The transgenic construct contains ZsYellow1 (from pZsYellow1-N1 (Clontech), a human codon-optimized yellow fluorescent protein from Zoanthus sp., under the control of the approximately 5kb mouse Cspg4 gene promoter and part of exon 1 of the Cspg4 gene (with a mutant ATG start codon).