aSMA-TK transgenic mice allow Ganciclovir-inducible ablation of proliferating cells (myofibroblasts) that express Acta2, and have applications in studies related to the role of aSMA+ fibroblasts in cancer progression and fibrosis.
Raghu Kalluri, University of Texas MD Anderson Cancer Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express a truncated TK, herpes simplex 1 virus thymidine kinase, gene under control of an extended 5.2kb mouse Acta2 (actin, alpha 2, smooth muscle, aorta) gene promoter that includes the Acta2 promoter plus exon 1, intron 1, and part of exon 2. This strain allows Ganciclovir-inducible ablation of proliferating cells (myofibroblasts) that express Acta2 . In vivo efficacy assessment (by immunofluorescence) of the aSMA-tk transgene in kidney tissue demonstrated dose-dependent Ganciclovir treatment (intraperitoneal injection) ablation of aSMA+ cells (proliferating interstitial myofibroblasts), with approximately 50% ablation. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (October 2017). During backcrossing, the Y chromosome may not have been fixed to the BALB/cAnNCrl genetic background.
The aSMA-TK transgenic mice are also available on a C57BL/6J congenic background (Stock No. 029921).
The aSMA-TK transgenic construct containing a truncated TK, herpes simplex 1 virus thymidine kinase, gene under control of an extended 5.2kb mouse Acta2 (actin, alpha 2, smooth muscle, aorta) gene promoter that includes the Acta2 promoter plus exon 1, intron 1, and part of exon 2, was microinjected into FVB fertilized mouse eggs. The founder line 1 was subsequently established. The mice were backcrossed to BALB/cAnNCrl for more than 10 generations. The mice were then backcrossed to BALB/cJ (Stock No. 000651) for 3 generations. During backcrossing, the Y chromosome may not have been fixed to the BALB/cAnNCrl genetic background.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize BALB/cByJ oocytes (Stock No. 001026).
Expressed Gene | TK, herpes simplex 1 virus thymidine kinase , herpes simplex 1 virus |
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Site of Expression | Myofibroblasts |
Allele Name | transgene insertion 1, Raghu Kalluri |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | alphaSMA-tk |
Gene Symbol and Name | Tg(Acta2-TK*)1Rkl, transgene insertion 1, Raghu Kalluri |
Gene Synonym(s) | |
Promoter | Acta2, actin, alpha 2, smooth muscle, aorta, mouse, laboratory |
Expressed Gene | TK, herpes simplex 1 virus thymidine kinase , herpes simplex 1 virus |
Site of Expression | Myofibroblasts |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgenic construct contains a truncated herpes simplex 1 virus thymidine kinase gene under control of an extended 5.2kb mouse Acta2 gene promoter that includes the Acta2 promoter plus exon 1, intron 1, and part of exon 2. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to BALB/cByJ inbred mice (Stock No. 001026). The Donating Investigator has not attempted to make the strain homozygous.
When using the aSMA-TK mouse strain in a publication, please cite the originating article(s) and include JAX stock #031155 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for Tg(Acta2-TK*)1Rkl |
Frozen Mouse Embryo | C.FVB-Tg(Acta2-TK*)1Rkl/J | $2595.00 |
Frozen Mouse Embryo | C.FVB-Tg(Acta2-TK*)1Rkl/J | $2595.00 |
Frozen Mouse Embryo | C.FVB-Tg(Acta2-TK*)1Rkl/J | $3373.50 |
Frozen Mouse Embryo | C.FVB-Tg(Acta2-TK*)1Rkl/J | $3373.50 |
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