This CRISPR-generated knock-out mutant of the UTP4 small subunit processome component (Utp4) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Utp4 encodes a transcriptional regulator that may be involved in the regulation of numerous viral promoters.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using CRISPR technology. The targeted gene, UTP4 small subunit processome component (Utp4), encodes a transcriptional regulator that may be involved in the regulation of numerous viral promoters. The alteration resulted in the deletion of 236 bp, which should result in the deletion of exon 4, amino acid change after residue 119, and early termination 17 amino acids later. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (CCAAAACAGCTTGTGAGACG, GAAGAAACGCTTGTGCATGG and ACAGCATATCCTATAGCCAC), designed to delete 236 bp in exon 4 of the UTP4 small subunit processome component (Utp4) gene, and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by PCR and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Utp4, UTP4 small subunit processome component|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 3 guide sequences CCAAAACAGCTTGTGAGACG, GAAGAAACGCTTGTGCATGG and ACAGCATATCCTATAGCCAC, which resulted in a 236 bp deletion beginning at Chromosome 8 positive strand position 106,898,379 bp, GCATGGGGGTATTAGTTCTG, and ending after GGACAGGATATTTTCCTGTG at 106,898,614 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000517277 (exon 4) and 151 bp of flanking intronic sequence including the splice acceptor and donor. In addition there is a 35 bp intronic deletion (TTCCCCGTCTCACAAGCTGTTTTGGTTTGTTTTGA, 8:106,898,306- 106,898,340) 38 bp before the 236 bp deletion that will not alter the result of the deletion. This mutation is predicted to cause a change of amino acid sequence after residue 119 and early truncation 17 amino acids later.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. As mice are characterized, data related to viability and fertility will be provided on the International Mouse Phenotyping Consortium website.
When using the C57BL/6NJ-Utp4em1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #42335 in your Materials and Methods section.