This CRISPR-generated knock-out mutant of the RAB10, member RAS oncogene family (Rab10) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Rab10 encodes a GTP-ase involved in the regulation of intracellular vesicle trafficking.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using CRISPR technology. The targeted gene, RAB10, member RAS oncogene family (Rab10), encodes a GTP-ase involved in the regulation of intracellular vesicle trafficking. The alteration resulted in the deletion of 452 bp, which should result in the deletion of exon 3, amino acid change after residue 62, and early termination 11 amino acids later. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (ATAAACTGTTTAGATTGTTG, ATAAGCTCCACACTTCATAG, GCTGAGCTCAAACTTTAGGA), designed to delete 452 bp in exon 3 of the RAB10, member RAS oncogene family (Rab10) gene, and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by PCR and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Rab10, RAB10, member RAS oncogene family|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 3 guide sequences ATAAACTGTTTAGATTGTTG, ATAAGCTCCACACTTCATAG, GCTGAGCTCAAACTTTAGGA, which resulted in a 452 bp deletion beginning at Chromosome 12 positive strand position 3,256,825 bp, ATTGTTGAGGGAGAGAGGGC, and ending after ATAAGCTCCACACTTCATAG at 3,257,276 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000462111 (exon 3) and 313 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 62 and early truncation 11 amino acids later.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. As mice are characterized, data related to viability and fertility will be provided on the International Mouse Phenotyping Consortium website.
When using the C57BL/6NJ-Rab10em1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #42330 in your Materials and Methods section.