This CRISPR-generated knock-out mutant of the zinc finger protein 706 (Zfp706) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Zfp706 encodes a transcription repressor involved in the exit of embryonic stem cells from self-renewal.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using CRISPR technology. The targeted gene, zinc finger protein 706 (Zfp706), encodes a transcription repressor involved in the exit of embryonic stem cells from self-renewal. The alteration resulted in the deletion of 629 bp, which should result in the deletion of exon 2, including the start site, and a null allele. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (GTGACTGTAGATAATATCCT, ACTGCTAGCTGGGAAAATGT, CCAGACAGTTCAAATATCAT and TATACTCATTTGTCATCTAG), designed to delete 629 bp in exon 2, including the start site, of the zinc finger protein 706 (Zfp706) gene, and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by PCR and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Zfp706, zinc finger protein 706|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences GTGACTGTAGATAATATCCT, ACTGCTAGCTGGGAAAATGT, CCAGACAGTTCAAATATCAT and TATACTCATTTGTCATCTAG, which resulted in a 629 bp deletion beginning at Chromosome 15 positive strand position 37,003,474 bp, CTACATTTTCCCAGCTAGCA, and ending after AGTTCAAATATCATTGGTAA at 37,004,102 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000514075 (exon 2) and 492 bp of flanking intronic sequence including the start site and is predicted to cause a null allele.|
When using the C57BL/6NJ-Zfp706em1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #42327 in your Materials and Methods section.