C.B6-Cysltr1^tm1Ykn/J

Stock number: 030960
Product name: CysLT₁R(-)
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These targeted mutant CysLT₁R(-) mice carry a knock-out allele of the mouse Cysltr1 gene (X-linked) that is useful in studies of vascular permeability in acute inflammatory responses.

Detailed description

Cysltr1 (cysteinyl leukotriene receptor 1; X-linked) is a receptor for cysteinyl leukotrienes (e.g. Ltc4s; see Stock No. 030813). The cysteinyl leukotrienes are proinflammatory lipid mediators generated by hematopoietic cells such as macrophages and mast cells in the mouse.

These mice carry a targeted knockout of the mouse gene in which exon 4, common to both long and short isoforms, was replaced with a neomycin cassette. Northern blot confirms the absence of both 1.4 and 4.0 kb mRNAs in lung.

Homozygotes are viable and fertile. Macrophages from these CysLT1R-deficient mice do not respond to either LTD4 or LTC4. Plasma protein extravasation, but not neutrophil infiltration, are significantly reduced in these CysLT1R-deficient mice subjected to zymosan A-induced peritoneal inflammation. Plasma protein extravasation is also significantly diminished in CysLT1R-deficient mice undergoing IgE-mediated passive cutaneous anaphylaxis as compared with the wild-type mice.

The phenotype of this BALB/c background strain is reported to be the same as that of the C57BL/6N genetic background line (see Stock No. 030814).

Development

A 278 bp coding region of exon 4, common to both the long and short isoforms of the X-linked Cysltr1 gene, was replaced with a neomycin resistance gene via homologous recombination in C57BL/6-derived V26.2 (ES-MK) embryonic stem (ES) cells. Resultant chimeric mice were bred to C57BL/6NCrl, then backcrossed to BALB/cAnNCrl for 10 generations by the donating laboratory.

Allele

Symbol: Cysltr1^tm1Ykn
Name: targeted mutation 1, Yoshihide Kanaoka
MGI accession ID: MGI:2182326
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: CysLT1R-; Cys-LT1R KO
Marker symbol: Cysltr1
Marker name: cysteinyl leukotriene receptor 1
Marker synonyms: Cyslt1; CysLT1R; CYSLTR; cysteinyl leukotriene 1 receptor; HMTMF81; leukotriene D4 receptor
Chromosome: X
Strain of origin: C57BL/6
Molecular note: A 278 bp coding region of exon 4 common to both the long and short isoforms of the gene was replaced with a neomycin resistance gene via homologous recombination. Northern blot analysis verified the absence of gene expression in lung of homozygous mutant animals.