These ACC1 flox conditional knockout mice possess loxP sites flanking exons 22-26 of the Acaca (acetyl-Coenzyme A carboxylase alpha) gene, a cytosolic enzyme involved in de novo lipogenesis. This strain may be useful for the study of lipogenesis and nonalcoholic fatty liver disease.
Dr. Jay D. Horton, Univ of Texas SW Med Center at Dallas
Acaca (ACC1 or acetyl-Coenzyme A carboxylase alpha) encodes a cytosolic enzyme that performs a rate limiting step in de novo lipogenesis. Two isozymes, ACC1 and ACC2, are the first committed enzymes in the de novo lipogenesis pathway.
When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 22-26 deleted in cre-expressing tissues.
When combined with mice carrying Acacbtm1.1Lowl (Stock No. 013042)
and Tg(Alb-cre)21Mgn (Stock No. 003574)
hepatocytes from progeny exhibit reduced hepatic steatosis, decreased polyunsaturated fatty acid (PUFA) concentrations in liver, plasma hypertriglyceridemia and increased VLDL secretion.
The targeting vector contains a loxP site inserted in intron 21 and a loxP-flanked neomycin cassette inserted in intron 26. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice from an unspecified background. The colony was then crossed to both B6N;129S-Acacbtm1.1Lowl/J (Stock No. 013042) and mice carrying the Tg(Alb-cre)21Mgn allele, these alleles were later bred out. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Jay D Horton|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Acaca, acetyl-Coenzyme A carboxylase alpha|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||The targeting vector contains a loxP site inserted in intron 21 and a loxP-flanked neomycin cassette inserted in intron 26.|
When maintaining a live colony, these mice are bred by homozygote sibling matings.
When using the ACC1 flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #030954 in your Materials and Methods section.
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