STOCK Srebf2^tm1Jdh/J

Stock number: 030826
Product name: Srebf-2^f
Research areas: Metabolism Research, Research Tools

Description

These Srebf2^fl conditional knock-out mice possess loxP sites flanking exon 1 of the sterol regulatory element binding factor 2 gene, a transcription factor involved in cholesterol biosynthesis.

Of note, a Srebf2^fl strain is available with the neo cassette removed as (Stock No. 031792).

Detailed description

These Srebf2^fl conditional knock-out mice possess loxP sites flanking exon 1 of the sterol regulatory element binding factor 2 gene, a bHLH transcription factor involved in cholesterol biosynthesis.

When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.

Progeny produced by combining Srebf2^fl mice and mice carrying the liver specific albumin-cre transgene (Tg(Alb-cre)21Mgn) exhibit decreased plasma and liver cholesterol concentrations as well as plasma and liver tryiglyceride levels.

Development

The targeting vector contains a loxP site inserted in the promoter region 660 bp upstream of exon 1, and a loxP site followed by a FRT-flanked neomycin cassette inserted into intron 1. The construct was electroporated into 129S6/SvEv derived SM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals crossed to C57BL/6J and offspring were crossed to C57BL/6J for an unknown number of generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Srebf2^tm1Jdh
Name: targeted mutation 1, Jay D Horton
MGI accession ID: MGI:5907035
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Synonyms: Srebf-2^flox
Marker symbol: Srebf2
Marker name: sterol regulatory element binding factor 2
Marker synonyms: bHLHd2; lop13; nuc; Srebf2_retired; SREBP2; SREBP-2; SREBP2gc
Chromosome: 15
Strain of origin: 129S6/SvEvTac
Molecular note: The targeting vector contains a loxP site inserted into the promoter region 660 bp upstream of exon 1 and a loxP site followed by a FRT-flanked neomycin cassette inserted into intron 1.