These targeted mutant CysLT1R(-) mice carry a knock-out allele of the mouse Cysltr1 gene (X-linked) that is useful in studies of vascular permeability in acute inflammatory responses.
Yoshihide Kanaoka, Brigham and Women's Hospital
Cysltr1 (cysteinyl leukotriene receptor 1; X-linked) is a receptor for cysteinyl leukotrienes (e.g. Ltc4s; see Stock No. 030813). The cysteinyl leukotrienes are proinflammatory lipid mediators generated by hematopoietic cells such as macrophages and mast cells in the mouse.
These mice carry a targeted knockout of the mouse gene in which exon 4, common to both long and short isoforms, was replaced with a neomycin cassette. Northern blot confirms the absence of both 1.4 and 4.0 kb mRNAs in lung.
Homozygotes are viable and fertile. Macrophages from these CysLT1R-deficient mice do not respond to either LTD4 or LTC4. Plasma protein extravasation, but not neutrophil infiltration, are significantly reduced in these CysLT1R-deficient mice subjected to zymosan A-induced peritoneal inflammation. Plasma protein extravasation is also significantly diminished in CysLT1R-deficient mice undergoing IgE-mediated passive cutaneous anaphylaxis as compared with the wild-type mice.
The phenotype of this C57BL/6N background strain is reported to be the same as that of the BALB/c genetic background line (see Stock No. 030960).
A 278 bp coding region of exon 4, common to both the long and short isoforms of the X-linked Cysltr1 gene, was replaced with a neomycin resistance gene via homologous recombination in C57BL/6-derived V26.2 (ES-MK) embryonic stem (ES) cells. This strain was crossed with inbred C57BL/6NCrl mice for 15 generations by the donating laboratory.
|Allele Name||targeted mutation 1, Yoshihide Kanaoka|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Yoshihide Kanaoka; Cysltr1tm1Ykn|
|Gene Symbol and Name||Cysltr1, cysteinyl leukotriene receptor 1|
|Gene Synonym(s)||CYSLT1; CYSLTR; cysteinyl leukotriene 1 receptor; CYSLT1R; expressed sequence BB147369; HMTMF81; leukotriene D4 receptor; BB147369; CysLT1R; Cyslt1|
|Strain of Origin||C57BL/6|
|Molecular Note||A 278 bp coding region of exon 4 common to both the long and short isoforms of the gene was replaced with a neomycin resistance gene via homologous recombination. Northern blot analysis verified the absence of gene expression in lung of homozygous mutant animals.|
Homozygotes, heterozygotes, and hemizygotes are viable and fertile.
When using the CysLT1R(-) mouse strain in a publication, please cite the originating article(s) and include JAX stock #030814 in your Materials and Methods section.
|Heterozygous or wildtype for Cysltr1<tm1Ykn>|
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