These mice carry a knock-out of the Ctgf gene. As part of an allelic set, with the Ctgf Lo-Hi allele (Stock No. 030767), Ctgf gene expression can be altered to range from 30% to 500% of wild-type (transcript) expression levels. These mice are suitable for use in applications related to the study of development, extracellular matrix homeostasis and fibrosis.
Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill
The targeted Ctgf gene encodes a connective tissue mitoattractant/mitogen secreted by vascular endothelial cells, that is important in extracellular matrix homeostasis, cell adhesion and chondrocyte proliferation and differentiation. Heterozygotes are viable and fertile. Homozygous null mice have a perinatal lethal phenotype, dying within 24 hours of birth.
Approximately 50% of wild-type levels of gene product (mRNA) are detected by qRT-PCR analysis of tail and kidney cortex of heterozygotes. Heterozgyotes exhibit lowered blood pressure when compared to wild-type controls. With streptozotocin-induced diabetes, heterozygotes have lower plasma triglyceride and plasma creatinine levels, decreased urinary albumin excretion and kidney mesangial expansion, as compared to controls.
A targeting vector with a neo cassette was used to disrupt exons 3 through 5. The construct was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into blastocysts.
The resulting chimeric animals were tested for germline transmission.
The mice were maintained on a 129S6 background.
To establish our live colony, an aliquot of frozen sperm was used to fertilize 129S1/SvImJ oocytes (Stock No. 002448).
|Allele Name||targeted mutation 1, Nobuyo Maeda|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Nobuyo Maeda; Ccn2tm1Mae|
|Gene Symbol and Name||Ccn2, cellular communication network factor 1|
|Gene Synonym(s)||Fisp12; NOV2; fibroblast inducible secreted protein; Fisp12; hypertrophic chondrocyte-specific gene product 24; IGFBP8; HCS24; CTGRP; Hcs24; CTGF|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A neomycin selection cassette replaced all of exons 3 through 5.|
When maintaining a live colony, heterozygous mice may be bred to wild-type siblings, or to 129S1/SvImJ inbred mice (Stock No. 002448). Homozygotes exhibit a perinatal lethal phenotype, dying within 24 hours of birth.
When using the Ctgf KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #030769 in your Materials and Methods section.
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