This CRISPR-generated knock-out mutant of the KRI1 homolog (Kri1) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Kri1 encodes a protein that may be involved in 40S ribosome biogenesis.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using CRISPR technology. The targeted gene, KRI1 homolog (Kri1), encodes a protein that may be involved in 40S ribosome biogenesis. The alteration resulted in the deletion of 356 bp, which should result in the deletion of exon 4, amino acid change after residue 92, and early termination 25 amino acids later. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (CGTCAGCTGCCAGTTCCCGA, ACAGGCCCTGAGCCACCCTC, GCAGAGAATGCAATAGGGCA and GGGCGAGTACCATATAGCCC), designed to delete 356 bp in exon 4 of the KRI1 homolog (Kri1) gene, and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by PCR and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Kri1, KRI1 homolog|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele from project Kri1-8530J-910M was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CGTCAGCTGCCAGTTCCCGA, ACAGGCCCTGAGCCACCCTC, GCAGAGAATGCAATAGGGCA and GGGCGAGTACCATATAGCCC, which resulted in a 356 bp deletion beginning at Chromosome 9 negative strand position 21,285,546 bp, GGGCTATATGGTACTCGCCC, and ending after AGTTCCCGAGGGACCCTGAG at 21,285,191 bp (GRCm38/mm10). This mutation deletes exon 4 and 229 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 92 and early truncation 25 amino acids later.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. As mice are characterized, data related to viability and fertility will be provided on the International Mouse Phenotyping Consortium website.
When using the C57BL/6NJ-Kri1em1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #42179 in your Materials and Methods section.