C.Cg-Rag1^tm1Mom Tg(DO11.10)10Dlo/J

Stock number: 030666
Product name: Rag1 DO11.10
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

 

These mice carry the MHC class II restricted rearranged T cell receptor transgene, Tg(DO11.10)10Dlo, to ovalbumin (OVA) peptide antigen, on a RAG1 deficient background.

Detailed description

Mice carrying the MHC class II restricted rearranged T cell receptor transgene, Tg(DO11.10)10Dlo, react to ovalbumin (OVA) peptide antigen. Use of this rearranged T cell receptor transgene requires H2^d background. These mice are RAG1 deficient, and should be maintained under SPF conditions.

Development

These mice were made at The Jackson Laboratory Repository by crossing C.Cg-Tg(DO11.10)10Dlo/J mice (Stock No. 003303) with C.129S7(B6)-Rag1^tm1Mom/J mice (Stock No. 003145). Mice are maintained on a congenic BALB/cJ (Stock No. 000651) background.

Tg(DO11.10)10Dlo transgene integration site: Gorham et al. 1996; PMID:8901605 identified the integration site of the DO11.10 transgenes to distal chromosome 18.

Allele

Symbol: Tg(DO11.10)10Dlo
Name: transgene insertion 10, Dennis Y Loh
MGI accession ID: MGI:2664398
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: CO11.10 TCR-alphabeta; D10; DO.10 Tg; DO.11; DO.11.10 TCR; DO11; DO11 TCR; DO11.10; DO-11.10; Do11.10 alphabeta TCR; Do11.10 alphabetaTCR; DO11.10 alphabeta-TCR; DO11.10 TCR; DO11.10 TCR Tg; DO11.10TCRalphabeta; DO-Tg; OVA-TCR^tg; Tg(DO11.10)Tcra/Tcrb; Tg(TcraDO11.10,TcrbDO11.10)10Dlo; TgN(DO11.10)10Dlo; TgN(DO11.10)10Loh
Marker symbol: Tg(DO11.10)10Dlo
Marker name: transgene insertion 10, Dennis Y Loh
Marker synonyms: CO11.10 TCR-alphabeta; DO.10 Tg; DO.11; DO.11.10 TCR; DO11; DO11 TCR; DO11.10; DO-11.10; Do11.10 alphabeta TCR; Do11.10 alphabetaTCR; DO11.10 alphabeta-TCR; DO11.10 TCR; DO11.10 TCR Tg; DO11.10TCRalphabeta; DO-Tg; OVA-TCR^tg; Tg(DO11.10)Tcra/Tcrb; TgN(DO11.10)10Dlo; TgN(DO11.10)10Loh
Chromosome: 18
Strain of origin: (C57BL/6 x C3H)F1 x BALB/c
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Site of expression: T cells
Molecular note: This double transgene contains rearranged Tcra and Tcrb genes and is expressed in the majority of T cells. These rearranged transgenes from the DO11.10 T cell hybridoma encode a chicken ovalbumin-specific MHC class II (I-A^d)-restricted T cell receptor. The allele was created by injection of a mix of the two separate transgenes Tg(TcraDO11.10)#Dlo and Tg(TcrbDO11.10)#Dlo into oocytes.
General note:

Mice carrying this pair of MHC class II restricted rearranged Tcra and Tcrb T cell receptor transgenes on a H2^d background react to ovalbumin (OVA) peptide antigen. Intraperitoneal administration of OVA to transgenic mice results in a rapid deletion of the immature CD4⁺ CD8⁺ TCR^lo thymocytes with progression to mature thymocytes. Apoptosis of cortical thymocytes in these mice occurs within 20 hours of treatment.

Allele

Symbol: Rag1^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857241
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Rag-; Rag^1tm1Mom; RAG-1-; Rag1^-; RAG1^null; Rag-1KO
Marker symbol: Rag1
Marker name: recombination activating gene 1
Marker synonyms: RAG-1; RNF74
Chromosome: 2
Strain of origin: 129S7/SvEvBrd-Hprt1⁺
Site of expression: expression is seen in bone marrow derived cell lines.
Molecular note: A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.