Ptch1lox/lox mice possess loxP sites flanking exon 3 of the patched 1 gene making them useful when studying embryonic development and tumorigenesis.
Robert J. Wechsler-Reya, Sanford Burnham Prebys Medical Discovery Institute
Ptch1lox/lox mice possess loxP sites flanking exon 3 of the patched 1 (Ptch1) gene. PTCH1 is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH), and desert hedgehog (DHH), and is involved in embryogenesis and tumorigenesis. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will express a truncated protein (13 amino acids after the first transmembrane domain) in the cre-expressing tissues.
When bred to STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J mice (Stock No. 007684), expressing tamoxifen-inducible Cre-recombinase under control of the Math1 promoter in neural progenitor cells, resulting offspring develop medulloblastoma by 3-6 months of age after tamoxifen-induction.
When bred to B6.Cg-Tg(Atoh1-cre)1Bfri/J mice (Stock No. 011104) and to FVB-Tg(CAG-luc,-GFP)L2G85Chco/J mice (Stock No. 008450), resulting offspring develop medulloblastoma by 2-3 months of age. All tissues, including tumors, express luciferase/GFP. When tumors are transplanted into naive mice, tumor growth can be monitored by bioluminescent imaging, and tumor cells can be isolated by flow cytometry.
The Ptch1lox/lox targeting vector was designed to insert loxP sites flanking exon 3, and a frt-flanked neomycin resistance (neo) cassette in intron 3 of the patched 1 (Ptch1) gene. The construct was electroporated into 129T2/SvEms-derived C1368 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred with C57BL/6J females. Resulting Ptch1lox/lox mice were bred once C57BL/6J mice and were further maintained on a mixed genetic background. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Brandon J Wainwright|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Ptc1lox; Ptch1c; Ptch1flox; Ptch1neo|
|Gene Symbol and Name||Ptch1, patched 1|
|Strain of Origin||129T2/SvEms|
|Molecular Note||Exon 3 was flanked by a single upstream loxP site in intron 2 and an FRT-neo-FRT-loxP cassette in intron 3. Mice carrying this allele were used to generate Ptchtm1.1Bjw.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Ptch1lox mouse strain in a publication, please cite the originating article(s) and include JAX stock #030494 in your Materials and Methods section.
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