Mist1nLacZKO mice have a β-gal gene and a neo cassette replacing the entire coding region of the basic helix-loop-helix family, member a15 (Bhlha15) gene, abolishing gene expression. These mice may be useful for studying chronic pancreatic injury and pancreatitis.
Stephen Konieczny, Purdue University
Mist1nLacZKO mice have a nuclear-localized β-gal gene (nLacZ) and a neo cassette replacing the entire coding region of the basic helix-loop-helix family, member a15 (Bhlha15) gene, abolishing gene expression. MIST1 is a basic helix-loop-helix transcription factor that is expressed in pancreatic acinar cells but not in duct, islet, or centroacinar cells. The Mist1 gene is also expressed in most serous secretory cells including acinar cells of the salivary gland, chief cells of the stomach, paneth cells in the small intestine and in lactating mammary aveolar cells. Homozygotes are viable and fertile. Beginning at E10.5, β-gal is seen in the primitive foregut, caudal, and medial to the forelimb bud, and the pancreatic bud. By E12.5, β-gal staining is seen in the two pancreatic buds in the lower part of the foregut. Staining in older embryos stains pancreatic acinar cells. At 12 months of age, focal lesions are evident in exocrine tissue, which progress and cause a significant decrease in the amount of acinar tissue along with large accumulations of other cell types. These mice show extensive disorganization of exocrine tissue and intracellular enzyme activation. The endocrine component of the pancreas appears unaffected in Mist1KO mice, and they contain normal levels of insulin and glucagon, and blood serum glucose levels are unaffected. Despite alterations in seminal fluid composition, male reproductive function and fertility are normal.
A targeting vector was designed to replace the entire coding region of the basic helix-loop-helix family, member a15 (Bhlha15) gene with a nuclear-localized β-galactosidase gene (nLacZ) fused to a neomycin resistance (neo) cassette in reverse orientation to the gene. The construct was electroporated into 129S6/SvEvTac-derived TL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. These Mist1nLacZKO mice were backcrossed to C57BL/6NHsd mice for at least 10 generations. Upon arrival at The Jackson Laboratory embryos were recovered to establish the colony.
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Site of Expression|
|Allele Name||targeted mutation 1, Stephen F Konieczny|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Stephen F Konieczny; Bhlha15tm1Skz|
|Gene Symbol and Name||Bhlha15, basic helix-loop-helix family, member a15|
|Gene Synonym(s)||BHLHB8; basic helix-loop-helix domain containing, class B, 8; 1810009C13Rik; RIKEN cDNA 1810009C13 gene; MIST1; Bhlhb8; 1810009C13Rik; bHlH; Mist1; Bhlhb8|
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||The entire coding region of the gene was replaced with a lacZ-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by immunohistochemical analysis, Western blot and RT-PCR.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Mist1nLacZKO mouse strain in a publication, please cite the originating article(s) and include JAX stock #030394 in your Materials and Methods section.
|Heterozygous or wildtype for Bhlha15<tm1Skz>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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