This CRISPR-generated knock-out mutant of the X-linked Kx blood group related 4 (Xkr4) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Xkr4 encodes a protein that may be involved in the regulation of thyroid-stimulating hormone levels.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using CRISPR technology. The targeted gene, X-linked Kx blood group related 4 (Xkr4), encodes a protein that may be involved in the regulation of thyroid-stimulating hormone levels. The alteration resulted in the deletion of 278 bp, which should result in the deletion of exon 2, amino acid change after residue 266, and early termination 26 amino acids later. As mice are characterized, phenotype data will be accessible on the International Mouse Phenotyping Consortium website.
Guide RNAs (CTCCCAATTCAGAAACCAAA, CCTCCAGCTGTCACTTTAAC, ACAGTCTTACTAGCTCAGTT and GTACGAAAGGAAAATGACTT), designed to delete 278 bp in exon 2 of the X-linked Kx blood group related 4 (Xkr4) gene, and Cas9 nuclease were introduced into C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by PCR and further bred to C57BL/6NJ (Stock No. 005304) to develop the colony.
|Allele Name||endonuclease-mediated mutation 1, Jackson|
|Allele Type||Endonuclease-mediated (Null/Knockout)|
|Gene Symbol and Name||Xkr4, X-linked Kx blood group related 4|
|Strain of Origin||C57BL/6NJ|
|Molecular Note||This allele from project Xkr4-8176J-F8924 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CTCCCAATTCAGAAACCAAA, CCTCCAGCTGTCACTTTAAC, ACAGTCTTACTAGCTCAGTT and GTACGAAAGGAAAATGACTT, which resulted in a 278 bp deletion beginning at Chromosome 1 negative strand position 3,421,962 bp, CTTTGGAGTTTGAATTTCAA, and ending after AAGGTAAGGATTTGCCATTT at 3,421,685 bp (GRCm38/mm10). This mutation deletes exon 2 and 78 bp of flanking intronic sequence including the splice acceptor and donor. In addition there is a 6 bp deletion (TTTAAC) in the intron 95 bp before the deletion that will not alter the effect of the exon deletion. This mutation is predicted to cause a change of amino acid sequence after residue 266 and early truncation 26 amino acids later.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. As mice are characterized, data related to viability and fertility will be provided on the International Mouse Phenotyping Consortium website.
When using the C57BL/6NJ-Xkr4em1(IMPC)J/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #42157 in your Materials and Methods section.