FVB/N-Tg(Cdx2-flpo)2Ddg/J

Stock number: 030288
Product name: Cdx2-NSE-FlpO
Research areas: Research Tools

Description

The Cdx2 promoter and neural specific enhancer (NSE) drive expression of Flp recombinase (FlpO) in the caudal neuroectoderm.

Detailed description

The Cdx2 (caudal type homeobox 2) promoter and neural specific enhancer (NSE) drive expression of Flp recombinase in this transgenic strain. Expression is seen in the spinal cord (neural ectoderm), but not in the brain, skin, or internal organs.

When bred with mice containing an FRT-flanked sequence of interest, Flp-mediated recombination will result in tissue-specific deletion of the FRT'ed region. This strain has been useful in defining the organizational logic of the spinal cord LTMR-RZ and its role in innocuous touch information processing and tactile perception.

Development

An 852 bp fragment of intron 1 of the mouse Cdx2 gene containing a neural specific enhancer was cloned upstream of a Flp recombinase (FlpO) cassette. The construct was injected into FVB oocytes. Resultant mice were maintained on an FVB/NJ genetic background by the donating laboratory. This is founder line 2.

Of note, for proper caudal neuroectoderm FlpO recombination, this line needs to be maintained on a background that is at least 50% FVB. This likely reflects the fact that regulatory sequences used to generate this transgenic line were cloned from FVB genomic DNA.

Allele

Symbol: Tg(Cdx2-flpo)2Ddg
Name: transgene insertion 2, David D Ginty
MGI accession ID: MGI:5828010
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: Cdx2-NSE-FlpO
Marker symbol: Tg(Cdx2-flpo)2Ddg
Marker name: transgene insertion 2, David D Ginty
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: FLP,FLP recombinase,Saccharomyces cerevisiae
Site of expression: Flp recombinase is expressed in neural ectoderm of the spinal cord.
Molecular note: An 852 bp fragment of intron 1 of the mouse Cdx2 gene containing a neural specific enhancer was cloned upstream of a Flp recombinase (FlpO) cassette.