This bacTRAP transgenic strain, incorporating an EGFP-L10a ribosomal fusion protein targeted to the Olig2 gene, enables cell labeling, sorting, and affinity purification of cell-specific polysomal RNAs.
Nathaniel Heintz, The Rockefeller University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
This bacTRAP line incorporates an EGFP-L10a ribosomal fusion protein targeted to the translational start site of the Olig2 (oligodendrocyte transcription factor 2) gene. The fusion protein enables affinity purification of CNS cell-specific polysomal RNAs which can be used for gene expression profiling.
Expression was carefully assayed by immunohistochemistry (IHC) with an antibody against enhanced green fluorescent protein (EGFP). The cerebellum, spinal cord, basal forebrain and corpus striatum, brainstem, and cerebral cortex were surveyed. Primary labeling was found to occur in mature oligodendrocytes and progenitors in the cortex and cerebellum. Fluorescence can also be visualized directly.
Images showing glial cell expression patterns may be found on the Gensat TRAP cell Showcase and Gensat gene expression pages.
A shuttle vector was used to introduce sequences encoding an EGFP-L10a ribosomal fusion protein into the translation start site of the Olig2 gene contained within BAC RP23-356P18. The modified BAC was introduced to FVB embryos. Founders and subsequent generations were bred to C57BL/6J mice for 2-5 generations by the donating laboratory. This is founder line JD97.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Rpl10a, ribosomal protein L10A, mouse, laboratory |
Site of Expression | EGFP labeling is observed in mature oligodendrocytes and progenitors. |
Allele Name | transgene insertion JD97, Nathaniel Heintz |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg(Olig2-EGFP/Rpl10a)JD97Htz; transgene insertion JD97, Nathaniel Heintz |
Gene Symbol and Name | Tg(Olig2-EGFP/Rpl10a)JD97Htz, transgene insertion JD97, Nathaniel Heintz |
Gene Synonym(s) | |
Promoter | Olig2, oligodendrocyte transcription factor 2, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Rpl10a, ribosomal protein L10A, mouse, laboratory |
Site of Expression | EGFP labeling is observed in mature oligodendrocytes and progenitors. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | Transgenic mice were generated using modified BAC Rp23-356P18. EGFP fused to the ribosomal protein L10a was inserted in the BAC in such a way that expression was driven by Olig2. |
Hemizygotes are viable and fertile. Homozygous viability/fertility is unknown.
When using the Olig2-EGFP/Rpl10a mouse strain in a publication, please cite the originating article(s) and include JAX stock #030265 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service | Genotype | Price |
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Hemizygous or non-carrier for Tg(Olig2-EGFP/Rpl10a)JD97Htz |
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
Frozen Mouse Embryo | B6;FVB-Tg(Olig2-EGFP/Rpl10a)JD97Htz/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Olig2-EGFP/Rpl10a)JD97Htz/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Olig2-EGFP/Rpl10a)JD97Htz/J | $3373.50 |
Frozen Mouse Embryo | B6;FVB-Tg(Olig2-EGFP/Rpl10a)JD97Htz/J | $3373.50 |
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