B6;FVB-Tg(Ntsr1-EGFP/Rpl10a)TS16Htz/J

Stock number: 030264
Product name: Ntsr1-EGFP/Rpl10a
Research areas: Neurobiology Research, Research Tools

Description

This bacTRAP transgenic strain, incorporating an EGFP-L10a ribosomal fusion protein targeted to the Ntsr1 gene, enables cell labeling, sorting, and affinity purification of cell-specific polysomal RNAs.

Detailed description

This bacTRAP line incorporates an EGFP-L10a ribosomal fusion protein targeted to the translational start site of the Ntsr1 (neurotensin receptor 1) gene. The fusion protein enables affinity purification of CNS cell-specific polysomal RNAs which can be used for gene expression profiling.

Expression was carefully assayed by immunohistochemistry (IHC) with an antibody against enhanced green fluorescent protein (EGFP). The cerebellum, spinal cord, basal forebrain and corpus striatum, brainstem, and cerebral cortex were surveyed. Primary labeling was found to occur in layer 6 corticothalamic pyramidal neurons of the cortex. Fluorescence can also be visualized directly.

Images showing gigantocellular neuron expression patterns may be found on the Gensat TRAP cell Showcase and Gensat gene expression pages.

Development

A shuttle vector was used to introduce sequences encoding an EGFP-L10a ribosomal fusion protein into the translation start site of the Ntsr1 gene contained within BAC RP23-314D14. The modified BAC was introduced to FVB embryos. Founders and subsequent generations were bred to C57BL/6J mice for an unknown number of generations by the donating laboratory. This is founder line TS16.

Allele

Symbol: Tg(Ntsr1-EGFP/Rpl10a)TS16Htz
Name: transgene insertion TS16, Nathaniel Heintz
MGI accession ID: MGI:5496691
Generation method: Transgenic
Attributes: Reporter
Synonyms: Ntsr1-bacTRAP; TS16
Marker symbol: Tg(Ntsr1-EGFP/Rpl10a)TS16Htz
Marker name: transgene insertion TS16, Nathaniel Heintz
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: Rpl10a,ribosomal protein L10A,mouse, laboratory; GFP,Green Fluorescent Protein,
Site of expression: EGFP labeling is observed in layer 6 corticothalamic pyramidal neurons.
Molecular note: Transgenic mice were generated using modified BAC Rp23-314D14. EGFP fused to the ribosomal protein L10a was inserted in the BAC in such a way that expression was driven by Ntsr1.