B6;FVB-Tg(Grp-EGFP/Rpl10a)JP25Htz/J

Stock number: 030259
Product name: Grp-EGFP/Rpl10a
Research areas: Neurobiology Research, Research Tools

Description

This bacTRAP transgenic strain, incorporating an EGFP-L10a ribosomal fusion protein targeted to the Grp gene, enables cell labeling, sorting, and affinity purification of cell-specific polysomal RNAs.

Detailed description

This bacTRAP line incorporates an EGFP-L10a ribosomal fusion protein targeted to the translational start site of the Grp (gastrin releasing peptide) gene. The fusion protein enables affinity purification of CNS cell-specific polysomal RNAs which can be used for gene expression profiling.

Expression was carefully assayed by immunohistochemistry (IHC) with an antibody against enhanced green fluorescent protein (EGFP). The cerebellum, spinal cord, basal forebrain and corpus striatum, brainstem, and cerebral cortex were surveyed. Primary labeling was found to occur in unipolar brush cells of the cerebellum (mGluR1 subtype). Minor labeling of cerebellar Bergman glia is also seen. Fluorescence can also be visualized directly.

Images showing CA3 pyramidal cell patterns may be found on the Gensat TRAP cell Showcase and Gensat gene expression pages.

Development

A shuttle vector was used to introduce sequences encoding an EGFP-L10a ribosomal fusion protein into the translation start site of the Grp gene contained within BAC RP23-179M10. The modified BAC was introduced to FVB embryos. Founders and subsequent generations were bred to C57BL/6J mice for an unknown number of generations by the donating laboratory. This is founder line JP25.

Allele

Symbol: Tg(Grp-EGFP/Rpl10a)JP25Htz
Name: transgene insertion JP25, Nathaniel Heintz
MGI accession ID: MGI:5496685
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Grp-EGFP/Rpl10a)JP25Htz
Marker name: transgene insertion JP25, Nathaniel Heintz
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: GFP,Green Fluorescent Protein,; Rpl10a,ribosomal protein L10A,mouse, laboratory
Site of expression: EGFP is expressed primarily in unipolar brush cells of the cerebellum (mGluR1 subtype).
Molecular note: Transgenic mice were generated using modified BAC Rp23-179M10. EGFP fused to the ribosomal protein L10a was inserted in the BAC in such a way that expression was driven by Grp.