This bacTRAP line incorporates an EGFP-L10a ribosomal fusion protein targeted to the translational start site of the Grm2 (glutamate receptor, metabotropic 2) gene. The fusion protein enables affinity purification of CNS cell-specific polysomal RNAs which can be used for gene expression profiling.

Expression was carefully assayed by immunohistochemistry (IHC) with an antibody against enhanced green fluorescent protein (EGFP). The cerebellum, spinal cord, basal forebrain and corpus striatum, brainstem, and cerebral cortex were surveyed. Primary labeling was found to occur in granule cell layer interneurons (inner golgi cells) of the cerebellum. Fluorescence can also be visualized directly.

Images showing neuron, golgi cell, and hippocampal granule neuron expression patterns may be found on the <a href="http://www.gensat.org/celltype_showcase.jsp?subtype_id=11&mouseType=TRAP "> Gensat TRAP cell Showcase </a> and <a href="http://www.gensat.org/TRAP_listing.jsp"> Gensat gene expression</a> pages.

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This bacTRAP transgenic strain, incorporating an EGFP-L10a ribosomal fusion protein targeted to the Grm2 gene, enables cell labeling, sorting, and affinity purification of cell-specific polysomal RNAs.

A minimum of two mice must be ordered. Additional mice will be charged on a per-mouse basis. Click the “Details” button to learn more.

Our minimum guarantee for cryorecovery orders is 2 carriers. To ensure that your colony can be rapidly expanded upon arrival, additional carriers may be added to your order.