B6;FVB-Tg(Cort-EGFP/Rpl10a)GM130Htz/J

Stock number: 030252
Product name: Cort-EGFP/Rpl10a
Research areas: Neurobiology Research, Research Tools

Description

This bacTRAP transgenic strain, incorporating an EGFP-L10a ribosomal fusion protein targeted to the Cort gene, enables cell labeling, sorting, and affinity purification of cell-specific polysomal RNAs.

Detailed description

This bacTRAP line incorporates an EGFP-L10a ribosomal fusion protein targeted to the translational start site of the Cort (cortistatin) gene. The fusion protein enables affinity purification of CNS cell-specific polysomal RNAs which can be used for gene expression profiling.

Expression was carefully assayed by immunohistochemistry (IHC) with an antibody against enhanced green fluorescent protein (EGFP). The cerebellum, spinal cord, basal forebrain and corpus striatum, brainstem, and cerebral cortex were surveyed. Primary labeling was found to occur in interneurons of the cortex. Fluorescence can also be visualized directly.

Images of transgene expression in brain may be found on the Gensat gene expression page.

Development

A shuttle vector was used to introduce sequences encoding an EGFP-L10a ribosomal fusion protein into the translation start site of the Cort gene contained within BAC RP23-281A14. The modified BAC was introduced to FVB embryos. Founders and subsequent generations were bred to C57BL/6J mice for 2 generations by the donating laboratory. This is founder line GM130.

Allele

Symbol: Tg(Cort-EGFP/Rpl10a)GM130Htz
Name: transgene insertion GM130, Nathaniel Heintz
MGI accession ID: MGI:5496681
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Cort-EGFP/Rpl10a)GM130Htz
Marker name: transgene insertion GM130, Nathaniel Heintz
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: GFP,Green Fluorescent Protein,; Rpl10a,ribosomal protein L10A,mouse, laboratory
Site of expression: EGFP is expressed primarily in interneurons of the cortex.
Molecular note: Transgenic mice were generated using modified BAC Rp23-281A14. EGFP fused to the ribosomal protein L10a was inserted in the BAC in such a way that expression was driven by Cort.