Extended synaptotagmin-like protein 1, 2 and 3 (Esyt1, Esyt2, Esyt3) are members of the multiple C2 domain membrane proteins. These proteins function as a tether between the endoplasmic reticulum (ER) and the plasma membrane (PM) interacting in a Ca2+-dependent manner with plasma membrane phospholipids to form the ER-plasma membrane junction. Mice homozygous for the all three ESYT null alleles are viable and fertile with a normal life expectancy and no observable pathology under normal conditions. Mouse embryo fibroblasts prepared from these mice exhibit a decrease in phosphatidylinositol 4,5 biphosphate (PIP2) transport and an impairment in Ca2+-induced ER-PM junction tightening. In the absence of Esyt1, Esyt2 and Esyt3 other tethering proteins are upregulated. This strain may be useful for studying homeostasis in plasma membrane phospholipids.

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&nbsp;Extended synaptotagmin-like protein 1, 2 and 3 (Esyt1, Esyt2, Esyt3)triple knock-out mice exhibit no observable phenotype under normal conditions. This strain may be useful for studying homeostasis in plasma membrane phospholipids.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.