Dkk2-/- mice have a neo cassette replacing all four coding exons encoding the dickkopf WNT signaling pathway inhibitor 2 (Dkk2) gene, abolishing gene expression. These mice may be useful for studying embryonic development through its role as an inhibitor of WNT signaling pathways.
Dianqing Wu, Yale University School of Medicine
Dkk2-/- mice have a neo cassette replacing all four coding exons encoding the dickkopf WNT signaling pathway inhibitor 2 (Dkk2) gene, abolishing gene expression. DKK2 is a secreted protein that inhibits Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye and bone formation. Mice homozygous for this allele are viable and fertile. Dkk2-/- osteoblast numbers are increased but are poorly mineralized in culture. They also have abnormal bone mineralization. Mice also display upper eyelid defects, including defective conjunctiva epithelium differentiation.
A targeting vector was designed to replace all four coding exons encoding the dickkopf WNT signaling pathway inhibitor 2 (Dkk2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129 embryonic stem (ES) cells. Correctly targeted ES cells were aggregated with C57BL/6NHsd embryos and they were implanted into foster female mice. The resulting chimeric males were bred to C57BL/6NHsd females and Dkk2-/- offspring were bred to C57BL/6NHsd mice for at least 10 generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ oocytes (Stock No. 005304).
|Allele Name||targeted mutation 1, Dianqing Wu|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Dkk2, dickkopf WNT signaling pathway inhibitor 2|
|Strain of Origin||129|
|Molecular Note||A neomycin resistance gene replaced the four coding exons via homologous recombination. RT-PCR failed to detect transcript in mutant bone marrow stromal osteoblasts, and western blot confirmed absence of protein in mutants.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Dkk2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #030130 in your Materials and Methods section.