These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Klf5 gene. This strain may be useful for generating conditional mutations in applications related to epithelial tissue development.
Dr. Jeffrey Whitsett, Children's Hospital Medical Center
The targeted Klf5 gene encodes a zinc finger protein that acts as a transcriptional activator and is expressed in epithelial cells.
These Klf5flox mice possess loxP sites flanking exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, however fewer than expected homozygotes are produced from heterozygous crosses and female homozygotes appear to be subfertile in our colony. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
Removal of the floxed sequence creates a null allele.
When bred to strains with inducible Cre recombinase expression in lung, this mutant mouse strain may be useful in studies of lung morphogenesis.
When bred to a strain with Cre recombinase expression in the embryonic endoderm, (see Stock No. 005622 for example), this mutant mouse strain may be useful in studies of the lung, intestine, and bladder epithelium during development.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a 3’ loxP site was inserted downstream of exon 3 of the targeted gene. A second loxP site was inserted upstream of exon 2. This construct was electroporated into unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were tested for germline transmission.
The chimeric animals were crossed to wildtype FVB/N mice. Heterozygotes were crossed to generate homozygotes. The mice were then crossed to other mutant lines of various genetic backgrounds.
Sperm from homozygous males on a genetically mixed background was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Jeffrey A Whitsett|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Klf5, Kruppel-like factor 5|
|Strain of Origin||Not Specified|
|Molecular Note||A loxP site was inserted upstream of exon 2 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 3.|
Heterozygotes may be bred to C57BL/6J (Stock# 000664), or wildtype littermates. While homozygotes are viable, fewer than expected homozygotes are produced (from heterozygous X heterozygous, and heterozygous x homozygous crosses) and female homozygotes appear to be subfertile in our colony.
When using the Klf5loxP mouse strain in a publication, please cite the originating article(s) and include JAX stock #029787 in your Materials and Methods section.