B6.Cg-Tg(Dmp1-cre/ERT2)D77Pdp/J

Stock number: 029594
Product name: 10kb-Dmp1-Cre-ERT2 transgenic line D77
Research areas: Cancer Research, Cell Biology Research, Developmental Biology Research, Internal/Organ Research, Neurobiology Research, Research Tools

Description

Stock No. 029594 has been discontinued. We recommend B6.Cg-Tg(Dmp1-cre/ERT2)0022Pdp/IkalJ (Stock No. 032757) as a replacement.

Allele

Symbol: Tg(Dmp1-cre/ERT2)D77Pdp
Name: transgene insertion D77, Paola Divieti Pajevic
MGI accession ID: MGI:5297120
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Synonyms: Dmp1-CreERt2
Marker symbol: Tg(Dmp1-cre/ERT2)D77Pdp
Marker name: transgene insertion D77, Paola Divieti Pajevic
Marker synonyms: Tg(Dmp1-cre/ERT2)1Pdp
Chromosome: UN
Strain of origin: (C57BL/6 x C3H)F1
Expressed genes: cre/ERT2,Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of expression: Tamoxifen-inducible Cre recombinase activity is expressed in osteocytes, some mature osteoblasts and bone lining cells.
Molecular note: A fragment (-9624 - +4439) of the Dmp1 gene containing a 9624 bp promoter region, the 95 bp exon 1, 4326 bp intron 1 and 17 bp of the initial non-coding region of exon 2 was cloned into a vector in front of the cre/ERT2 cDNA. The linearized vector was injected into B6C3F1 pronuclei. 14 pups that were positive for cre integration were identified and six independent founders were crossed to wild-type B6 mice. Two founders demonstrated penetrant excision of the STOP cassette when crossed to Rosa26-lacZ reporter mice, and were analyzed in greater detail. Two 10kb-Dmp1-Cre-ERT2 transgenic founder lines (D77 and 0022) were identified to have tamoxifen-inducible Cre recombinase activity in both osteocytes and mature osteoblasts. The two lines were similar with the only difference that line D77 had greater expression in mature osteoblasts.