STOCK Or8a1^tm26Mom Tg(Or8a1,-MAPT/mCherry)5MGaFeins/J

Stock number: 029429
Product name: MouSensor SuperSniffer 5●M71Cα
Strain synonyms: 5.M71Calpha
Research areas: Neurobiology Research, Research Tools, Sensorineural Research

Description

These 5●M71Cα “MouSensor” mice express an IRES-tau-mCherry tagged mouse Or8a1 (formerly Olfr151), olfactory receptor family 1 subfamily A member 1, gene, and the Or8a1^tm26Mom allele. These mice may be useful in studies related to in vivo olfactory neuronal morphology visualization, odorant receptor coding and olfaction.

Detailed description

These 5●M71Cα mutant mice express the an IRES-tau-mCherry tagged mouse Or8a1 (formerly Olfr151), olfactory receptor 151, gene in approximately 3-6% of the olfactory sensory neurons. Mice hemizygous for the transgene and homozygous for the Or8a1^tm26Mom GFP knock-in allele are viable and fertile. Transgene expression is detected in the main olfactory epithelium. Hemizgyotes have approximately 16 copies of the transgene. The Donating Investigator has not attempted to make the strain homozygous for the transgene. Homozygous viability/fertility has not been tested for the transgene (January 2017).

Development

The nx21-OR-IRES-Reporter MouSensors transgenic vector is designed to allow insertion of a 21-bp singular gene enhancer, odorant receptor coding sequence, and IRES-Reporter cassette. The 5●M71Cα transgene was generated from the nx21-OR-IRES-Reporter MouSensors transgenic vector and contains 5 repeats of the 21bp enhancer sequence (from the olfactory receptor H-region enhancer sequence), and an IRES-tau-mCherry tagged mouse Or8a1, olfactory receptor family 1 subfamily A member 1, gene. The 5●M71Cα transgene was injected into fertilized CBA X C57BL/6 mouse eggs. Founder line 4MGa (5MG alpha) was subsequently established. The mice were then crossed to B6;129P2-Or8a1^tm26Mom/MomJ mice (Stock No. 006676). Hemizgyotes have approximately 16 copies of the transgene. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Or8a1^tm26Mom
Name: targeted mutation 26, Peter Mombaerts
MGI accession ID: MGI:3051336
Generation method: Targeted
Attributes: Reporter
Synonyms: M71-G; M71-GFP; M71-IRES-tauGFP; Olfr7^tm2Mom
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Marker synonyms: GA_x6K02T2PVTD-31408136-31407207; M71; MOR171-2; Olfr151; Olr1195; OR11-318; OST025
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151.
Molecular note: An IRES-tauGFP gene was inserted 3 nucleotides downstream of the endogenous stop codon. Crossing with EIIa-Cre mice excised a floxed neo at the 3' end of the inserted cassette.

Allele

Symbol: Tg(Or8a1,-MAPT/mCherry)5MGaFeins
Name: transgene insertion 5MGa, Paul Feinstein
MGI accession ID: MGI:5819067
Generation method: Transgenic
Attributes: Reporter; Inserted expressed sequence
Synonyms: 5*M71Calpha; 5*M71Calpha MouSensor; Tg(Olfr151,-MAPT/mCherry)5MGaFeins
Marker symbol: Tg(Or8a1,-MAPT/mCherry)5MGaFeins
Marker name: transgene insertion 5MGa, Paul Feinstein
Chromosome: UN
Strain of origin: CBA x C57BL/6
Expressed genes: Or8a1,olfactory receptor family 8 subfamily A member 1,mouse, laboratory; RFP,Red Fluorescent Protein,coral
Site of expression: This transgene is expressed in the main olfactory epithelium.
Molecular note: The transgene contains 485 bp of the Olfr151 promoter (M71 minigene)and 5 repeats of the 21 bp (5x21) enhancer sequence from the olfactory receptor H-region upstream of the transcriptional start site followed by an IRES-tau-mCherry tagged Olfr151 gene. This is founder line 5MGalpha. Sixteen copies of the transgene integrated into this line. Transgene expression is detected in 3-6% of the olfactory sensory neurons.