Exons 20-23 of the mouse Piezo1 gene are flanked by loxP sites in this conditional targeted mutant strain. Cre recombinase-mediated excision of the floxed region results a knock-out allele useful in studies of vascular development and red blood cell volume.
Ardem Patapoutian, The Scripps Research Institute
Genetic Background | Generation |
---|---|
?+pN1F15
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed)) | Piezo1 | piezo-type mechanosensitive ion channel component 1 |
Piezo1 (piezo-type mechanosensitive ion channel component 1), expressed in endothelial cells of developing blood vessels in mice, is required for vascular development. It encodes a mechanosensitive non-selective cation channel that, when activated, plays an essential role in red blood cell (RBC) volume homeostasis.
Exons 20-23 of the mouse Piezo1 gene are flanked by loxP sites in this conditional targeted mutant strain. Cre recombinase-mediated excision of the floxed region results in a frameshift and produces a knock-out allele.
Crosses with Vav1-iCre mice (Stock No. 008610) delete PIEZO1 protein expression in the hematopoietic system. Lymphocytes from the compound mutant mice exhibit greater than 95% deletion of Piezo1 transcript.
This strain was created using the Piezo1tm1a(KOMP)Wtsi Wellcome Trust Sanger Institute KOMP (Knockout Mouse Project) targeting construct.
An FRT-lacZ-loxP-neo-FRT-loxP cassette was introduced to intron 19, and a loxP site was placed in intron 23 via homologous recombination in B6.Cg-Thy1a-derived Bruce 4 embryonic stem (ES) cells. Resultant chimeric mice were bred to C57BL/6J animals. Crosses with Flp mice excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites.
Allele Name | targeted mutation 2.1, Ardem Patapoutian |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed)) |
Allele Synonym(s) | P1f |
Gene Symbol and Name | Piezo1, piezo-type mechanosensitive ion channel component 1 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 8 |
Molecular Note | The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. The FRT-lacZ-loxP-neo-FRT-loxP cassette is introduced into intron 19 followed by a loxP site placed in intron 23. Flp-mediated recombination excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites. |
Heterozygous and homozygous floxed mice are viable and fertile.
When using the P1f mouse strain in a publication, please cite the originating article(s) and include JAX stock #029213 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Piezo1<tm2.1Apat> |
Frozen Mouse Embryo | STOCK Piezo1<tm2.1Apat>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Piezo1<tm2.1Apat>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Piezo1<tm2.1Apat>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Piezo1<tm2.1Apat>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.