B6.Cg-Piezo1^tm2.1Apat/J

Stock number: 029213
Product name: P1^f
Research areas: Research Tools

Description

Exons 20-23 of the mouse Piezo1 gene are flanked by loxP sites in this conditional targeted mutant strain. Cre recombinase-mediated excision of the floxed region results a knock-out allele useful in studies of vascular development and red blood cell volume.

Detailed description

Piezo1 (piezo-type mechanosensitive ion channel component 1), expressed in endothelial cells of developing blood vessels in mice, is required for vascular development. It encodes a mechanosensitive non-selective cation channel that, when activated, plays an essential role in red blood cell (RBC) volume homeostasis.

Exons 20-23 of the mouse Piezo1 gene are flanked by loxP sites in this conditional targeted mutant strain. Cre recombinase-mediated excision of the floxed region results in a frameshift and produces a knock-out allele.

Crosses with Vav1-iCre mice (Stock No. 008610) delete PIEZO1 protein expression in the hematopoietic system. Lymphocytes from the compound mutant mice exhibit greater than 95% deletion of Piezo1 transcript.

Development

This strain was created using the Piezo1^{tm1a(KOMP)Wtsi} Wellcome Trust Sanger Institute KOMP (Knockout Mouse Project) targeting construct.

An FRT-lacZ-loxP-neo-FRT-loxP cassette was introduced to intron 19, and a loxP site was placed in intron 23 via homologous recombination in B6.Cg-Thy1^a-derived Bruce 4 embryonic stem (ES) cells. Resultant chimeric mice were bred to C57BL/6J animals. Crosses with Flp mice excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites.

Allele

Symbol: Piezo1^tm2.1Apat
Name: targeted mutation 2.1, Ardem Patapoutian
MGI accession ID: MGI:5766345
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Piezo1
Marker name: piezo-type mechanosensitive ion channel component 1
Chromosome: 8
Strain of origin: B6.Cg-Thy1^a
Molecular note: The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. The FRT-lacZ-loxP-neo-FRT-loxP cassette is introduced into intron 19 followed by a loxP site placed in intron 23. Flp-mediated recombination excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites.