Exons 20-23 of the mouse Piezo1 gene are flanked by loxP sites in this conditional targeted mutant strain. Cre recombinase-mediated excision of the floxed region results a knock-out allele useful in studies of vascular development and red blood cell volume.
Ardem Patapoutian, The Scripps Research Institute
Piezo1 (piezo-type mechanosensitive ion channel component 1), expressed in endothelial cells of developing blood vessels in mice, is required for vascular development. It encodes a mechanosensitive non-selective cation channel that, when activated, plays an essential role in red blood cell (RBC) volume homeostasis.
Exons 20-23 of the mouse Piezo1 gene are flanked by loxP sites in this conditional targeted mutant strain. Cre recombinase-mediated excision of the floxed region results in a frameshift and produces a knock-out allele.
Crosses with Vav1-iCre mice (Stock No. 008610) delete PIEZO1 protein expression in the hematopoietic system. Lymphocytes from the compound mutant mice exhibit greater than 95% deletion of Piezo1 transcript.
This strain was created using the Piezo1tm1a(KOMP)Wtsi Wellcome Trust Sanger Institute KOMP (Knockout Mouse Project) targeting construct.
An FRT-lacZ-loxP-neo-FRT-loxP cassette was introduced to intron 19, and a loxP site was placed in intron 23 via homologous recombination in B6.Cg-Thy1a-derived Bruce 4 embryonic stem (ES) cells. Resultant chimeric mice were bred to C57BL/6J animals. Crosses with Flp mice excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites.
|Allele Name||targeted mutation 2.1, Ardem Patapoutian|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Gene Symbol and Name||Piezo1, piezo-type mechanosensitive ion channel component 1|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. The FRT-lacZ-loxP-neo-FRT-loxP cassette is introduced into intron 19 followed by a loxP site placed in intron 23. Flp-mediated recombination excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites.|
Heterozygous and homozygous floxed mice are viable and fertile.
When using the P1f mouse strain in a publication, please cite the originating article(s) and include JAX stock #029213 in your Materials and Methods section.