C57BL/6-Tg(Csf1r-cre)1Mnz/J

Stock number: 029206
Product name: Csfr1^Cre
Research areas: Research Tools

Description

Csfr1^Cre BAC transgenic mice express cre recombinase from the mouse colony stimulating factor 1 receptor promoter. They enable excision of floxed genomic segments in conventional dendritic cells (cDCs).

Detailed description

Csfr1^Cre BAC transgenic mice express Cre recombinase from the mouse Csfr1 (colony stimulating factor 1 receptor) promoter.

Crosses with fluorescent floxed stop reporter mice demonstrate expression in pre-dendritic cells and conventional dendritic cells (cDCs), plasmacytoid dendritic cells (pDCs), monocytes and macrophages. The percentage of labeled pDCs is lower than in cDCs, macrophages and monocytes. Although cre expression is not specific for cDCs, cDCs are efficiently targeted in these mice (near 100% recombination efficiency), therefore they are considered suitable for cDC targeting.

An inducible in vivo system for cDC depletion can be generated by crossing these mice with zDC-loxStoplox-DTR (see Stock No. 028539; also called zDC^lSlDTR) animals. zDC^lSlDTR mice express human diptheria toxin receptor (DTR) under the control of the Zbtb46 promoter after the excision of a floxed Stop signal and the administration of diptheria toxin (DT). Sixteen hours after DT administration, cDCs (but not monocytes, macrophages, or CD8⁺ T or B cells) are efficiently depleted in the compound mutant mice.

Development

IRES-cre was introduced to the 3' UTR of the mouse Csf1r gene encoded by BAC RP23-174D18, right after the endogenous Stop codon in exon 21. The BAC was microinjected into C57BL/6 mouse oocytes and founders were bred to C57BL/6J by the donating laboratory.

Allele

Symbol: Tg(Csf1r-cre)1Mnz
Name: transgene insertion 1, Michel C Nussenzweig
MGI accession ID: MGI:5763756
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Csf1r-cre)1Mnz
Marker name: transgene insertion 1, Michel C Nussenzweig
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recomnbinase is expressed in pre-dendritic cells and conventional dendritic cells (cDCs), plasmacytoid dendritic cells (pDCs), monocytes and macrophages.
Molecular note: The trasngene was generated by inserting IRES-Cre into the 3' UTR of the mouse Csf1r gene encoded by BAC RP23-174D18 right after the endogenous stop codon in exon 21. Line and founder numbers were not specified.