C57BL/6-Tg(CD2-Ccr7)1Adl/J

Stock number: 029095
Product name: CCR7 Tg
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

CCR7 Tg transgenic mice express Ccr7 cDNA in T lymphocytes under control of a human CD2 promoter. These mice may be useful in applications related to T cell exit from peripheral tissue.

Detailed description

CCR7 Tg transgenic mice have a human CD2 antigen (CD2) promoter directing expression of a chemokine (C-C motif) receptor 7 (Ccr7) cDNA. CCR7 is a G protein-coupled receptor involved in the migration of memory T cells to from peripheral tissues into draining lymph nodes. Homozygous CCR7 Tg mice are viable and fertile. CD4⁺ and CD8⁺ T cells from the lymph nodes of CCR7 Tg transgenic mice have slightly elevated expression of CCR7. In a model of allergic airway inflammation, CCR7⁺ effector T cells enter inflamed tissue and continue to migrate into the lymph system, whereas CCR7^- T cells accumulate in the inflamed lung.

Development

The CCR7 Tg transgene was designed with a human CD2 antigen (CD2) promoter directing expression of a chemokine (C-C motif) receptor 7 (Ccr7) cDNA. The transgene was microinjected into fertilized C57BL/6 oocytes, and mice from founder line 1 were bred to C57BL/6NTac mice to establish a colony of CCR7 Tg mice. Mice were maintained by breeding to C57BL/6 mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Tg(CD2-Ccr7)1Adl
Name: transgene insertion 1, Andrew D Luster
MGI accession ID: MGI:5770558
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(CD2-Ccr7)1Adl
Marker name: transgene insertion 1, Andrew D Luster
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Ccr7,chemokine (C-C motif) receptor 7,mouse, laboratory
Molecular note: The transgene was designed with a human CD2 antigen (CD2) promoter directing expression of a chemokine (C-C motif) receptor 7 (Ccr7) cDNA.