Mcp-5- mice lack expression of chemokine (C-C motif) ligand 12 Ccl12. These mice may be useful for studying monocyte mobilization from bone marrow and recruitment to inflammatory sites.
Andrew D Luster, Massachusetts General Hospital-East
Mcp-5- mutant mice previously had a floxed neo cassette disrupting the coding region of the chemokine (C-C motif) ligand 12 (Ccl12 or Mcp-5) gene. The insertion of the neo cassette also inhibited expression of MCP-5 and MCP-2 (chemokine (C-C motif) ligand 8 (Ccl8), which lies 12kb downstream of Mcp-5. Mcp-2-/Mcp-5- mice are available as Stock No. 029088. To create Mcp-5- mice (Stock No. 029089), Mcp-2-/Mcp-5- mice were bred to mice expressing Cre Recombinase to remove the neo cassette, and restore expression of MCP-2 mice cre-expressing tissues of the offspring. These mice are viable and fertile as homozygotes. Ccl12 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils to sites of injury or infection through association with CCR2 (chemokine (C-C motif) receptor 2). Mcp-2-/Mcp-5- mice are more susceptible to Listeria monocytogenes infection than wild-type mice. They have normal numbers of monocytes in bone marrow and in blood.
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette into the coding region of the chemokine (C-C motif) ligand 12 (Ccl12) gene. The construct was electroporated into 129S4/SvJae-derived RF8 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6. Subsequently, mice were bred to mice expressing Cre Recombinase to delete the neo cassette. Offspring were crossed to remove the cre expressing transgene. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Israel F Charo|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Ccl12 KO; Mcp-5-|
|Gene Symbol and Name||Ccl12, chemokine (C-C motif) ligand 12|
|Strain of Origin||129S4/SvJae|
|Molecular Note||The floxed neo was removed from the chemokine (C-C motif) ligand 12 locus, which restored expression of chemokine (C-C motif) ligand 8 only.|
When maintaining a live colony homozygous mice may be bred together.
When using the Mcp-5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #029089 in your Materials and Methods section.