These floxed mutant mice possess loxP sites flanking exons 2-14 of the Dvl2 gene. This strain may be useful for generating conditional mutations in studies of neurodevelopment and planar cell polarity.
Dr. Anthony Wynshaw-Boris, Case Western Reserve University, School of Medicine
William Snider, University of North Carolina, Chapel Hill
The protein encoded by the Dvl2 gene is a dishevelled protein family member, which are involved in Wnt signaling pathways. Dishevelled segment polarity protein 2 has a critical role in medial-lateral polarity/planar cell polarity.
These mice possess loxP sites flanking exons 2 through 14 of the targeted Dvl2 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 14 deleted in the cre-expressing tissues.
Removal of the floxed sequence creates a null allele.
When bred to a strain with Cre recombinase expression primarily in the central nervous system, this mutant mouse strain may be useful in studies of ependymal cell rotational and translational polarity.
A targeting vector containing a FRT site flanked Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a 5’loxP site was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted into exon 15, distal to the stop codon. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to mice expressing FLP recombinase (Stock No. 003946) to excise the FRT site flanked NEO cassette. Mice that retained the loxP site flanked exons 2-14 were then bred to remove the FLP recombinase allele.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, William D Snider|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Allele Synonym(s)||Dvl2flox; Dvl2tm2.1Awb|
|Gene Symbol and Name||Dvl2, dishevelled segment polarity protein 2|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||An FRT flanked neo cassette and loxP site were inserted upstream of exon 2 and a loxP site was inserted into exon 15 distal to the stop codon. Flp mediated recombination removed the neo cassette.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Dvl2flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #029061 in your Materials and Methods section.