B6;129-Pbrm1^tm1Zhwa/J

Stock number: 029049
Product name: BAF180^f
Research areas: Cell Biology Research, Research Tools

Description

BAF180^f/f floxed mice have loxP sites flanking exon 11 of the polybromo 1 (Pbrm1) gene. This strain may be useful for generating conditional mutations for use in applications related to BAF180-mediated chromatin remodeling and cell cycle regulation.

Detailed description

BAF180^f/f floxed mice possess loxP sites flanking exon 11 of the polybromo 1 (Pbrm1) gene. BAF180 is a subunit of an ATP-dependent BAF chromatin remodeling complex, in the SNF/SWI superfamily. BAF180 is required for ligand-dependent transcriptional activation. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 11 deleted in cre-expressing tissues.

Development

A targeting vector was designed to insert a single loxP site upstream of exon 11, and an FRT-flanked βgeo cassette, followed by a loxP site, downstream of exon 11 of the polybromo 1 (Pbrm1) gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric BAF180^f/f mice were maintained on a mixed B6;129 genetic background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Pbrm1^tm1Zhwa
Name: targeted mutation 1, Zhong Wang
MGI accession ID: MGI:5810066
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Pbrm1
Marker name: polybromo 1
Chromosome: 14
Strain of origin: 129P2/OlaHsd
Molecular note: A targeting vector is designed to insert a single loxP site upstream of exon 11, and an FRT-flanked betageo cassette, followed by a loxP site, downstream of exon 11.