BAF180f/f floxed mice have loxP sites flanking exon 11 of the polybromo 1 (Pbrm1) gene. This strain may be useful for generating conditional mutations for use in applications related to BAF180-mediated chromatin remodeling and cell cycle regulation.
Zhong Wang, University of Michigan
BAF180f/f floxed mice possess loxP sites flanking exon 11 of the polybromo 1 (Pbrm1) gene. BAF180 is a subunit of an ATP-dependent BAF chromatin remodeling complex, in the SNF/SWI superfamily. BAF180 is required for ligand-dependent transcriptional activation. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 11 deleted in cre-expressing tissues.
A targeting vector was designed to insert a single loxP site upstream of exon 11, and an FRT-flanked βgeo cassette, followed by a loxP site, downstream of exon 11 of the polybromo 1 (Pbrm1) gene. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric BAF180f/f mice were maintained on a mixed B6;129 genetic background.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Zhong Wang|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Allele Synonym(s)||BAF180fl; Pbrm1tm1.1Zhwa|
|Gene Symbol and Name||Pbrm1, polybromo 1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A targeting vector is designed to insert a single loxP site upstream of exon 11, and an FRT-flanked betageo cassette, followed by a loxP site, downstream of exon 11.|
When maintaining a live colony mice homozygous for the floxed allele may be bred together.
When using the BAF180f mouse strain in a publication, please cite the originating article(s) and include JAX stock #029049 in your Materials and Methods section.