This CRISPR/Cas9 generated Chchd10del10 allele has a 10 nucleotide deletion mutation. These mice may be useful in studies related to mitochondrial bioenergetics and pathogenesis of diseases such as mitochondrial myopathy, motor neuron disease, frontotemporal dementia, Parkinson’s disease, and amyotrophic lateral sclerosis.
Cathleen Lutz, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Endonuclease-mediated (Not Specified) | Chchd10 | coiled-coil-helix-coiled-coil-helix domain containing 10 |
CRISPR/cas9 endonuclease mediated genome editing of the Chchd10, coiled-coil-helix-coiled-coil-helix domain containing 10, gene was used to introduce a 10 nucleotide deletion mutation. The targeted Chchd10 gene encodes a mitochondrial intermembrane protein that is involved with mitochondrial cristae morphology and oxidative phosphorylation. Mutations in the human CHCHD10 gene can cause frontotemporal dementia and amyotrophic lateral sclerosis (ALS). Homozygotes are viable and fertile. As the mice are characterized, we will modify the strain description and add phenotype data.
Plasmids encoding a signal guide RNA designed to introduce a 10 nucleotide deletion in the Chchd10 gene and the cas9 nuclease were introduced into the cytoplasm C57BL/6NJ-derived fertilized eggs with well recognized pronuclei. Correctly targeted embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and PCR and further bred to C57BL/6NJ (Stock No. 005304) for 2 generations to develop the colony.
Allele Name | endonuclease-mediated mutation 11, Cathy Lutz |
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Allele Type | Endonuclease-mediated (Not Specified) |
Allele Synonym(s) | |
Gene Symbol and Name | Chchd10, coiled-coil-helix-coiled-coil-helix domain containing 10 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NJ |
Chromosome | 10 |
Molecular Note | CRISPR/Cas9 genome editing is used to introduce a 10 nucleotide deletion. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Chchd10 del10
mouse strain in a publication, please cite the originating article(s) and include JAX stock #029033 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Chchd10<em11(del10)Lutzy> |
Frozen Mouse Embryo | C57BL/6NJ-Chchd10<em11Lutzy>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6NJ-Chchd10<em11Lutzy>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6NJ-Chchd10<em11Lutzy>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6NJ-Chchd10<em11Lutzy>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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