These TRE/TrfT mice express human transferrin under direction of A tet-responsive element (TRE; tetO). These mice may be useful when studying iron transport.
Brian Popko, University of Chicago
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
TRE/TrfT mice have a tet-responsive element (TRE; tetO) placed upstream of the human transferrin (TRF) cDNA and a β-globin polyadenylation signal. Mice hemizygous for this transgene are viable and fertile. The donating investigator has not attempted to make this strain homozygous. TRF is an iron-binding blood plasma glycoprotein that is involved in the transport of iron from sites of absorption and heme degradation to those of storage and utilization. When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), transferrin expression in the resulting double mutant offspring can be regulated with tetracycline or its analog doxycycline (dox).
A tet-responsive element (TRE; tetO) was placed upstream of the human transferrin (TRF) cDNA and a β-globin polyadenylation signal in a transgenic construct introduced into fertilized C57BL/6J oocytes. TRE/TrfT mice from founder line 29 were maintained by breeding to C57BL/6J mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | TF, transferrin, human |
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Site of Expression | When bred with another mouse expressing tTA or rtTA, transferrin expression in the resulting double mutant offspring can be regulated with tetracycline or its analog doxycycline (dox). |
Allele Name | transgene insertion 29, Brian Popko |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | TRE/TrfT |
Gene Symbol and Name | Tg(tetO-TRF)29Pop, transgene insertion 29, Brian Popko |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | TF, transferrin, human |
Site of Expression | When bred with another mouse expressing tTA or rtTA, transferrin expression in the resulting double mutant offspring can be regulated with tetracycline or its analog doxycycline (dox). |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | A tet-responsive element was placed upstream of the human transferrin (TRF) cDNA and a beta-globin polyadenylation signal. |
When maintaining a live colony hemizygous mice may be bred together. The donating investigator has not attempted to make this strain homozygous.
When using the TRE/TrfT mouse strain in a publication, please cite the originating article(s) and include JAX stock #029021 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for Tg(tetO-TRF)29Pop |
Frozen Mouse Embryo | C57BL/6J-Tg(tetO-TRF)29Pop/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(tetO-TRF)29Pop/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Tg(tetO-TRF)29Pop/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Tg(tetO-TRF)29Pop/J | $3373.50 |
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