APOE*2 KI are CRISPR/Cas9-generated mice with R130C and R176C point mutations in the human APOE sequence of an existing humanized ApoE knock-in allele and express the human APOE isoform E2.
Mike Sasner, The Jackson Laboratory
Genetic Background | Generation |
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?+pN1
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Allele Type | Gene Symbol | Gene Name |
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Endonuclease-mediated (Inserted expressed sequence, Humanized sequence) | Apoe | apolipoprotein E |
Starting at:
$255.00 Domestic price for female 4-week |
510.00 Domestic price for breeder pair |
The targeted Apoe gene encodes apolipoprotein E, which is important in lipoprotein metabolism and cardiovascular disease as well as Alzheimer's disease, immunoregulation and cognition. CRISPR/Cas9 was used to introduce a C to T nucleotide substitution point mutation in amino acids R130C and R176C into the human APOE sequence in the Apoetm1.1(APOE*4)Adiuj allele. The resulting APOE*2 KI mice express APOE isoform 2, from the endogenous Apoe locus. The APOE 2 isoform is found in approximately 7% of the population, and does not bind to cell surface receptors as well as the 3 and 4 isoforms.
As the mice are characterized, we will modify the strain description and add phenotype data. Homozygous viability/fertility has not been tested (June 2018).
A mutagenic oligo based approach was used to generate this strain. Plasmids encoding a signal guide RNA designed to introduce the R130C and R176C point mutations into the Apoetm1.1(APOE*4)Adiuj allele and the cas9 nuclease were introduced into the cytoplasm of B6(SJL)-Apoetm1.1(APOE*4)Adiuj/J (Stock No. 027894)-derived fertilized eggs with well recognized pronuclei. Correctly targeted embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and PCR and further bred to C57BL/6J (Stock No. 000664) to develop the colony.
Expressed Gene | APOE, apolipoprotein E, human |
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Site of Expression |
Allele Name | endonuclease-mediated mutation 3, MODEL-AD Center |
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Allele Type | Endonuclease-mediated (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Apoe, apolipoprotein E |
Gene Synonym(s) | |
Expressed Gene | APOE, apolipoprotein E, human |
Strain of Origin | B6(SJL)-Apoetm1.1(APOE*4)Adiuj/J |
Chromosome | 7 |
Molecular Note | CRISPR/Cas9 genome editing methodologies was used to introduce R130C and R176C point mutations into human APOE protein carried by the Apoetm1.1(APOE*4)Adpmc allele. |
Heterozygotes may be bred to C57BL/6J (Stock No. 000664), or wildtype littermates. As the mice are characterized, we will modify the strain description if necessary and add data related to viability and fertility. Homozygous viability/fertility has not been tested (June 2018).
When using the APOE2 KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #029017 in your Materials and Methods section.
Service/Product | Description | Price |
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Heterozygous or wildtype for Apoe<em3(APOE*)Adiuj> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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