Overview

Also Known As: APOE*2 KI, APOE2 KI

APOE*2 KI are CRISPR/Cas9-generated mice with R130C and R176C point mutations in the human APOE sequence of an existing humanized ApoE knock-in allele and express the human APOE isoform E2.

Donating Investigator

Mike Sasner, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Apoe^{em3(APOE)Adiuj}*

Allele Type	Gene Symbol	Gene Name
Endonuclease-mediated (Inserted expressed sequence, Humanized sequence)	Apoe	apolipoprotein E

View Genetics

Research Applications

Metabolism Research
Neurobiology Research
Cardiovascular Research
Research Tools

View All Research Applications

Details

Detailed Description

The targeted Apoe gene encodes apolipoprotein E, which is important in lipoprotein metabolism and cardiovascular disease as well as Alzheimer's disease, immunoregulation and cognition. CRISPR/Cas9 was used to introduce a C to T nucleotide substitution point mutation in amino acids R130C and R176C into the human APOE sequence in the Apoe^{tm1.1(APOE*4)Adiuj} allele. The resulting APOE*2 KI mice express APOE isoform 2, from the endogenous Apoe locus. The APOE 2 isoform is found in approximately 7% of the population, and does not bind to cell surface receptors as well as the 3 and 4 isoforms.

As the mice are characterized, we will modify the strain description and add phenotype data. Homozygous viability/fertility has not been tested (June 2018).

Development

A mutagenic oligo based approach was used to generate this strain. Plasmids encoding a signal guide RNA designed to introduce the R130C and R176C point mutations into the Apoe^{tm1.1(APOE*4)Adiuj} allele and the cas9 nuclease were introduced into the cytoplasm of B6(SJL)-Apoe^{tm1.1(APOE*4)Adiuj}/J (Stock# 027894)-derived fertilized eggs with well recognized pronuclei. Correctly targeted embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and PCR and further bred to C57BL/6J (Stock No. 000664) to develop the colony.

Expression Data

Expressed Gene	APOE, apolipoprotein E, human
Site of Expression

Control Suggestions

Wild-type from the colony

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Apoe^{em3(APOE)Adiuj}*

Allele Symbol: Apoe^{em3(APOE)Adiuj}*

Allele Name	endonuclease-mediated mutation 3, MODEL-AD Center
Allele Type	Endonuclease-mediated (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	endonuclease-mediated mutation 3, MODEL-AD Center; Apoe^{em3(APOE*)Adiuj}
Gene Symbol and Name	Apoe, apolipoprotein E
Gene Synonym(s)	expressed sequence AI255918; APO-E; AD2; LPG; AI255918; LDLCQ5; APOEA; ApoE4
Expressed Gene	APOE, apolipoprotein E, human
Strain of Origin	B6(SJL)-Apoe^{tm1.1(APOE*4)Adiuj}/J
Chromosome	7
Molecular Note	CRISPR/Cas9 genome editing methodologies was used to introduce R130C and R176C point mutations into human APOE protein carried by the Apoe^{tm1.1(APOE*4)Adpmc} allele.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Metabolism Research
- Lipid Metabolism
Neurobiology Research
- Alzheimer's Disease
  - APOE mutants
Cardiovascular Research
- Other
Research Tools
- Cardiovascular Research
- Neurobiology Research

Mammalian Phenotype Terms by Genotype

References

Additional - Apoe^{em3(APOE)Adiuj}* related

Technical Support

Contact Technical Support

Genotyping Protocols
- High Resolution Melting: Apoe^{tm1.1(APOE*4)Adiuj}
- Sanger sequencing: Apoe^{tm1.1(APOE*4)Msasn-E4/E3/E2-Alternate 1}
- Genotyping resources and troubleshooting
Breeding Considerations

Heterozygotes may be bred to C57BL/6J (Stock# 000664), or wildtype littermates.
As the mice are characterized, we will modify the strain description if necessary and add data related to viability and fertility. Homozygous viability/fertility has not been tested (June 2018).
- Additional Breeding and Husbandry Support
Citation
When using the APOE2 KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #029017 in your Materials and Methods section.

Strain Interest Registration

Please fill out the form below to indicate your interest in purchasing this JAX®Mice strain.
This information helps us manage the colony build and better meet the broad needs of the research community.
Please send any technical questions to Technical Support.

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Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

The use of this mouse model is subject to the terms and conditions of the Limited License from The Broad Institute.

The use of this mouse model is subject to the terms and conditions of the Limited Use Label License from Caribou Biosciences, Inc.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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Also Known As: APOE*2 KI, APOE2 KI

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Apoeem3(APOE*)Adiuj

Allele Symbol: Apoeem3(APOE*)Adiuj

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Apoeem3(APOE*)Adiuj related

Genotyping Protocols

Breeding Considerations

Citation

Strain Interest Registration

Contact Information

Interest

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Apoe^{em3(APOE)Adiuj}*

Allele Symbol: Apoe^{em3(APOE)Adiuj}*

Additional - Apoe^{em3(APOE)Adiuj}* related