These transgenic animals carry the HA tagged mouse DNA-damage-inducible transcript 4-like (Ddit4l) driven by a conditional mouse Myh6-tetO cardiac-specific promoter. When crossed with a tTA/rtTA strain, these mice may be useful in studies of cardiomyopathy.
Saumya Das, Massachusetts General Hospital
Genetic Background | Generation |
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|
Allele Type |
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Transgenic (Inducible, Inserted expressed sequence, Epitope tag) |
The DNA-Damage-Inducible Transcript 4-Like protein encoded by the Ddit4l gene is involved in the mTOR signalling pathway and thus, autophagy.
In these transgenic mice the HA-DDiT4L transgene expression is regulated by the tetracycline-responsive regulatory element (tetO).
When crossed with a tTA, tetracycline transactivator (tTA; "Tet-off") strain, human influenza hemagglutinin tagged Ddit4l transgene expression can be regulated with the tetracycline analog, doxycycline, in the resulting double mutant mice. Mutant mice expressing the HA-DDiT4L transgene from birth exhibit mild ventricular remodeling and baseline systolic cardiac dysfunction. Homozygotes are viable and fertile. The Donating Investigator has not compared the phenotypes between hemizygous and homozygous animals.
When crossed with a myocardial specific tTA, tetracycline transactivator (tTA; "Tet-off"), strain, with removal of doxycycline treatment the resulting double transgenic mice exhibit cardiac DDIT4L levels similar to that observed after TAC (transverse aortic constriction) induced hypertrophy and increased autophagy. Upon withdrawal of doxycycline, transgene expression is detected in heart, but not in lung, skeletal muscle, and liver.
A transgenic construct containing the mouse DNA-damage-inducible transcript 4-like (Ddit4l) gene, tagged with human influenza hemagglutinin, under the control of the tetO/Myh6, tet-operator (tetracycline-responsive element) and mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (alpha-MHC) fusion promoter, was injected into fertilized FVB mouse eggs. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).
Expressed Gene | HA, influenza hemagglutinin, |
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Expressed Gene | Ddit4l, DNA-damage-inducible transcript 4-like, mouse, laboratory |
Site of Expression | Cardiac muscle. |
Allele Name | transgene insertion 1, Saumya Das |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence, Epitope tag) |
Allele Synonym(s) | DDiT4L; HA-DDiT4L |
Gene Symbol and Name | Tg(Myh6/tetO-Ddit4l)1Sdas, transgene insertion 1, Saumya Das |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory |
Expressed Gene | HA, influenza hemagglutinin, |
Expressed Gene | Ddit4l, DNA-damage-inducible transcript 4-like, mouse, laboratory |
Site of Expression | Cardiac muscle. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgenic construct contains the mouse DNA-damage-inducible transcript 4-like (Ddit4l) gene, tagged with human influenza hemagglutinin, under the control of the tetO/Myh6, tet-operator (tetracycline-responsive element) and mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (alpha-MHC) fusion promoter. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the FVB/N-Tg(Myh6/tetO-Ddit4l)1Sdas/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #028973 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(Myh6/tetO-Ddit4l)1Sdas |
Frozen Mouse Embryo | FVB/N-Tg(Myh6/tetO-Ddit4l)1Sdas/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Myh6/tetO-Ddit4l)1Sdas/J | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tg(Myh6/tetO-Ddit4l)1Sdas/J | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tg(Myh6/tetO-Ddit4l)1Sdas/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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