This CRISPR/Cas9-generated mutant of the Card14 gene carries an insertion of a single nucleotide [C] (indel) in exon 6 that results in a frameshift.
Mark Mellett, University Hospital Zurich
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Endonuclease-mediated (Not Specified) | Card14 | caspase recruitment domain family, member 14 |
This CRISPR/Cas9-generated point mutant of the Card14 (caspase recruitment domain family, member 14) carries an insertion of a single nucleotide [C] in exon 6, resulting in a frameshift. The Card14 gene encodes a scaffold protein that is a regulator of apoptosis and may have a role in innate immunity. Mutations in this gene, specifically of the single glutamic acid (E138) in the coiled-coil domain, have been associated with psoriasis and pityriasis rubra pilaris. As the mice are characterized, we will modify the strain description and add phenotype data. Homozygous viability/fertility has not been tested (June 2018).
Creation and development of this strain was supported by the Rare Genomics Institute's BeHEARD(Helping Empower and Accelerate Research Discoveries) Initiative. The Jackson Laboratory was a 2016/2017 BeHEARD Technology Sponsor.
Plasmids encoding a single guide RNA designed to insert a single nucleotide [C] in exon 6 of the Card14 gene and the cas9 nuclease were introduced into the cytoplasm C57BL/6J-derived fertilized eggs with well recognized pronuclei. Correctly targeted embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and PCR and further bred to C57BL/6J (Stock No. 000664) to develop the colony. Sperm from N2 mice was cryopreserved.
Allele Name | endonuclease-mediated mutation 10, Cathy Lutz |
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Allele Type | Endonuclease-mediated (Not Specified) |
Allele Synonym(s) | |
Gene Symbol and Name | Card14, caspase recruitment domain family, member 14 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 11 |
Molecular Note | CRISPR/Cas9 genome editing was used to to insert a single nucleotide (C) into exon 6 causing a frameshift. |
Heterozygotes may be bred to C57BL/6J (Stock No. 000664), B6129SF1/J (Stock No. 101043), or wildtype littermates. As the mice are characterized, we will modify the strain description if necessary and add data related to viability and fertility. Homozygous viability/fertility has not been tested (June 2018).
When using the Card14ins1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #028901 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Card14<em10(ins1)Lutzy> |
Frozen Mouse Embryo | C57BL/6J-Card14<em10(ins1)Lutzy>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Card14<em10(ins1)Lutzy>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Card14<em10(ins1)Lutzy>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Card14<em10(ins1)Lutzy>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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