These knock-in, knock-out mice express human MBL2 from the endogenous mouse Mbl1 locus and may be useful in studies related to host defense, ischemia/reperfusion, innate immunity and the complement pathway.
Gregory L. Stahl, Brigham and Women’s Hospital, Harvard Medical School
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout, Inserted expressed sequence) | Mbl1 | mannose-binding lectin (protein A) 1 |
The Soluble Mannose-Binding Lectin (Protein C) 2, encoded by MBL2, is part of the complement system of innate immunity, recognizing and binding to mannose and N-acetylglucosamine found on microorganisms, and can function as an acute phase protein. In mice, mannose-binding lectin is encoded by 2 genes, Mbl1 and Mbl2.
These mice express a knock-in, knock-out allele in which human MBL2 cDNA replaced part of exon 2 and all of exons 3 and 4 of the endogenous mouse Mbl1 gene.
Mice that are homozygous for the targeted mutation are viable and fertile.
When crossed to MBL-null mice, B6.129S4-Mbl1tm1Kata Mbl2tm1Kata/J (Stock No. 006122), the resulting triple mutant mice exhibit serum MBL2 protein levels that is approximately half the level found in inbred C57BL/6J mice, and lectin pathway activation similar to that observed in wildtype control mice. Induced hyperglycemia in the triple mutant mice results in increased serum MBL2 protein levels.
A targeting vector containing human MBL2 cDNA and a FRT site flanked NEO cassette was used to disrupt part of exon 2 and all of exons 3 and 4 of the endogenous Mbl1 gene. The construct was electroporated into unspecified C57BL/6 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting male chimeric animals were tested for germline transmission and bred to FLP recombinase expressing mice on an unknown background.
Mice that no longer carried the NEO cassette were then bred to B6.129S4-Mbl1tm1Kata Mbl2tm1Kata/J mice (Stock No. 006122).
The mice were then bred to remove the Mbl1tm1Kata Mbl2tm1Kata alleles.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | MBL2, mannose binding lectin 2, human |
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Site of Expression |
Allele Name | targeted mutation 1.1, Gregory L Stahl |
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Allele Type | Targeted (Null/Knockout, Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Mbl1, mannose-binding lectin (protein A) 1 |
Gene Synonym(s) | |
Expressed Gene | MBL2, mannose binding lectin 2, human |
Strain of Origin | C57BL/6 |
Chromosome | 14 |
Molecular Note | A human MBL2 cDNA and FRT flanked neo cassette replaced a genomic region encompassing part of exon 2 and all of exons 3 and 4 via homologous recombination. Flp mediated recombination removed the neo cassette. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Mbl1 MBL2 KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #028892 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Mbl1<tm1.1(MBL2)Glst> |
Frozen Mouse Embryo | STOCK Mbl1<tm1.1(MBL2)Glst>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Mbl1<tm1.1(MBL2)Glst>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Mbl1<tm1.1(MBL2)Glst>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Mbl1<tm1.1(MBL2)Glst>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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