STOCK Mbl1^{tm1.1(MBL2)Glst}/J

Stock number: 028892
Product name: Mbl1 MBL2 KI
Research areas: Cardiovascular Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These knock-in, knock-out mice express human MBL2 from the endogenous mouse Mbl1 locus and may be useful in studies related to host defense, ischemia/reperfusion, innate immunity and the complement pathway.

Detailed description

The Soluble Mannose-Binding Lectin (Protein C) 2, encoded by MBL2, is part of the complement system of innate immunity, recognizing and binding to mannose and N-acetylglucosamine found on microorganisms, and can function as an acute phase protein. In mice, mannose-binding lectin is encoded by 2 genes, Mbl1 and Mbl2. These mice express a knock-in, knock-out allele in which human MBL2 cDNA replaced part of exon 2 and all of exons 3 and 4 of the endogenous mouse Mbl1 gene. Mice that are homozygous for the targeted mutation are viable and fertile.

When crossed to MBL-null mice, B6.129S4-Mbl1^tm1Kata Mbl2^tm1Kata/J (Stock No. 006122), the resulting triple mutant mice exhibit serum MBL2 protein levels that is approximately half the level found in inbred C57BL/6J mice, and lectin pathway activation similar to that observed in wildtype control mice. Induced hyperglycemia in the triple mutant mice results in increased serum MBL2 protein levels.

Development

A targeting vector containing human MBL2 cDNA and a FRT site flanked NEO cassette was used to disrupt part of exon 2 and all of exons 3 and 4 of the endogenous Mbl1 gene. The construct was electroporated into unspecified C57BL/6 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting male chimeric animals were tested for germline transmission and bred to FLP recombinase expressing mice on an unknown background. Mice that no longer carried the NEO cassette were then bred to B6.129S4-Mbl1^tm1Kata Mbl2^tm1Kata/J mice (Stock No. 006122). The mice were then bred to remove the Mbl1^tm1Kata Mbl2^tm1Kata alleles. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Mbl1^{tm1.1(MBL2)Glst}
Name: targeted mutation 1.1, Gregory L Stahl
MGI accession ID: MGI:5705816
Generation method: Targeted
Attributes: Null/Knockout; Inserted expressed sequence
Marker symbol: Mbl1
Marker name: mannose-binding lectin (protein A) 1
Chromosome: 14
Strain of origin: C57BL/6
Expressed genes: MBL2,mannose binding lectin 2,human
Molecular note: A human MBL2 cDNA and FRT flanked neo cassette replaced a genomic region encompassing part of exon 2 and all of exons 3 and 4 via homologous recombination. Flp mediated recombination removed the neo cassette.