C.FVB-Tg(Mt1-OVA)1Akab/J

Stock number: 028851
Product name: mtOVA
Research areas: Research Tools

Description

These transgenic mice express a soluble form of chicken ovalbumin under the control of the mouse Mt1 promoter. Protein can be detected circulating in the serum.

Detailed description

These mtOVA transgenic mice express a serum-soluble form of chicken ovalbumin (sOVA) under the regulatory control of the mouse Mt1 (metallothionein 1) promoter.

Adoptive transfer of OVA-specific T cells from DO11 mice (see Stock No. 003303) into the mtOVA mice have been used to study immune responses to self-antigen. They were instrumental in demonstrating that B7 molecules promote effective T cell priming and contain effector responses when self-antigens are presented as foreign.

Development

Chicken ovalbumin peptide (OVA_323-339) is expressed under the control of the mouse Mt1 (metallothionein 1) promoter in this transgenic strain. This line was backcrossed to BALB/cJ for more than 10 generations by the donating investigator.

Allele

Symbol: Tg(Mt1-OVA)1Akab
Name: transgene insertion 1, Abul Abbas
MGI accession ID: MGI:4442200
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Mt1-OVA)1Akab
Marker name: transgene insertion 1, Abul Abbas
Chromosome: UN
Strain of origin: FVB
Expressed genes: OVA,ovalbumin,chicken
Site of expression: T-cells.
Molecular note: A soluble ovalbumin was expressed under the control of the metallothionein promoter.