C57BL/6-Lrrk2^tm3.1Arte/DralJ

Stock number: 028801
Product name: LRRK2 S910A,S935A KI
Strain synonyms: constitutive LRRK2[S910A+S935A] knockin
Research areas: Neurobiology Research

Description

These LRRK2 S910A S935A knock-in mice carry 2 point mutations in exon 21 of the Lrrk2 gene and are suitable for use in applications related to the study of Parkinson's disease.

Detailed description

Mutations in the human LRRK2 gene have been found to cause autosomal dominant Parkinson disease-8. These knock-in mice carry the S910A S935A point mutations in exon 21 of the Lrrk2 gene. Mutant LRRK2 transgene expression results in decreased expression of total LRRK2 protein levels in kidney, but not in brain or lung tissues, as determined by Western blot analysis. (Tissues examined: lung, kidney, brain) Mice that are homozygous for the targeted mutation are viable and fertile. No obvious overt phenotype is detected in homozygotes. As the mice are further characterized, we will modify the strain description and add phenotype data.

Development

A targeting vector containing a FRT site flanked PuroR selection cassette was used to insert S910A and S935A point mutations into exon 21 of the Lrrk2 gene. The construct was electroporated into C57BL/6NTac-derived Art B6 3.6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to C57BL/6-Tg(CAG-Flpe)2Arte mice to remove the FRT site flanked selection cassette, and then bred to remove the transgene. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Lrrk2^tm3.1Arte
Name: targeted mutation 3.1, TaconicArtemis
MGI accession ID: MGI:5811334
Generation method: Targeted
Attributes: Not Applicable
Marker symbol: Lrrk2
Marker name: leucine-rich repeat kinase 2
Chromosome: 15
Strain of origin: C57BL/6NTac
Molecular note: A targeting vector containing an FRT-flanked PuroR selection cassette was used to insert S910A and S935A point mutations into exon 21. Flp-mediated recombination removed the FRT-flanked PuroR cassette.