These floxed mutant mice possess loxP sites flanking exon 2 of the Becn1 gene. This strain may be useful for generating conditional mutations in applications related to the regulation of autophagy, tumorigenesis, tumor progression and neurodegeneration.
Zhenyu Yue, Mount Sinai School of Medicine
Beclin 1, encoded by the Becn1 gene, plays a central role in autophagy regulation and is involved in early embryonic development, tumorigenesis, tumor progression and neurodegeneration. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
Removal of the floxed sequence creates a null allele.
When bred to a strain with Cre recombinase expression in Purkinje cells (see Stock No. 004146 for example), or other neurons, this mutant mouse strain may be useful in applications related to neurodegeneration.
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into unspecified embryonic stem (ES) cells derived from C57BL/6J-Tyrc-2J/J. Correctly targeted ES cells were injected into blastocysts.
The resulting chimeric animals were crossed to flipper mice (on an unknown genetic background) expressing FLP recombinase to excise the FRT flanked selection cassette.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1.1, Zhenyu Yue|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Becn1, beclin 1, autophagy related|
|Strain of Origin||C57BL/6J-Tyrc-2J|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette and left exon 2 floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Becn1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #028794 in your Materials and Methods section.