B6.129-Grm5^tm1.1Jixu/J

Stock number: 028626
Product name: mGluR5^loxP
Research areas: Neurobiology Research, Research Tools

Description

Exon 7 of the mouse Grm5 gene is flanked by loxP sites. Cre-mediated excision of the exon results in a knockout allele.

Detailed description

Grm5 (glutamate receptor, metabotropic 5; also called mGluR5) has been demonstrated to have important roles in several forms of synaptic plasticity and learning behaviors, and has been suggested as a potential therapeutic target in several neurological disorders.

Exon 7 of the mouse Grm5 gene is flanked by loxP sites in this mGlur5^loxP strain. Cre-mediated excision of the floxed exon results in a knockout allele. Homozygous floxed mice are viable and fertile, and show no overt phenotype.

Homozygous knockout mice generated through crosses with Prm-cre animals (in which cre recombinase is expressed in the male germline) show a partial impairment in the acquisition of fear conditioning. More markedly, extinction of both contextual and auditory fear is completely abolished.

Development

A cassette containing a neomycin resistance (neo) gene, flanked by loxP sites, under the control of the phosphoglycerol kinase (PGK) promoter was introduced into the intron 720 bp downstream of exon 7, which is 940 bp in length. An additional loxP site was placed 450 bp upstream of exon 7. The mutation was created via homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Resultant chimeric animals were crossed with C57BL/6 mice then Prm-cre animals to excise the neomycin cassette. This strain was backcrossed to C57BL/6 for more than 10 generations by the donating lab.

Allele

Symbol: Grm5^tm1.1Jixu
Name: targeted mutation 1.1, Jian Xu
MGI accession ID: MGI:3840366
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Grm5
Marker name: glutamate receptor, metabotropic 5
Chromosome: 7
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A floxed neo cassette was inserted into intron 7, 720 bp downstream of exon 7, and a third loxP site was inserted upstream of exon 7 via homologous recombination. The neo cassette was then removed via Cre mediated recombination.