This bacTRAP line expresses EGFP-RPL10a from the mouse Hcrt promoter. Ribosome-bound mRNAs from Hcrt-expressing neurons of the hypothalamus can be purified using EGFP as an affinity tag.
Nathaniel Heintz, The Rockefeller University
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
Hypocretin (orexin; Hcrt)-containing neurons of the hypothalamus are essential for the normal regulation of sleep and wake behaviors and have been implicated in feeding, anxiety, depression, and reward.
This bacTRAP line expresses EGFP-RPL10a from the mouse Hcrt promoter. Ribosome-bound mRNAs from Hcrt-expressing neurons of the hypothalamus can be purified using EGFP as an affinity tag. GFP expression, as determined by confocal immunofluorescent microscopy, is reported in greater than 85% of neurons with detectable Hcrt expression and there are no instances of EGFP-RPL10a expression in Hcrt-negative cells. No EGFP-RPL10a expression is reported elsewhere in the adult brain.
EGFP-RPL10a was inserted at the translation start site of the Hcrt gene encoded by BAC RP23-258L05. The modified BAC was injected into FVB eggs. Resultant founder mice (founder line JD218) were crossed with C57BL/6J animals for 3 or 4 generations by the donating lab.
Expressed Gene | EGFP/Rpl10, enhanced green fluorescent protein; ribosomal protein 10, |
---|---|
Site of Expression | EGFP/Rpl10a fusion protein is expressed in neurons with detectable Hcrt expression. |
Allele Name | transgene insertion JD218, Joseph D Dougherty |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Hcrt-EGFP/Rpl10a)JD218Jdd, transgene insertion JD218, Joseph D Dougherty |
Gene Synonym(s) | |
Promoter | Hcrt, hypocretin, mouse, laboratory |
Expressed Gene | EGFP/Rpl10, enhanced green fluorescent protein; ribosomal protein 10, |
Site of Expression | EGFP/Rpl10a fusion protein is expressed in neurons with detectable Hcrt expression. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | BAC RP23-258L05, was modified by insertion of an EGFP/RPL10a fusion construct at the translation start site of the Hcrt gene. |
Hemizygotes are viable and fertile. Homozygotes are also viable and have proven fertile in limited tests by the donating lab.
When using the Hcrt bacTRAP mouse strain in a publication, please cite the originating article(s) and include JAX stock #028619 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(Hcrt-EGFP/Rpl10a)JD218Jdd |
Frozen Mouse Embryo | B6;FVB-Tg(Hcrt-EGFP/Rpl10a)JD218Jdd/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Hcrt-EGFP/Rpl10a)JD218Jdd/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Hcrt-EGFP/Rpl10a)JD218Jdd/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;FVB-Tg(Hcrt-EGFP/Rpl10a)JD218Jdd/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.