Overview

Also Known As:NSG FcRn-/- hFcRn (276) Tg

NSG FcRn-/- hFcRn (276) Tg mice are NOD.scid.Il2Rγc^null ("NSG") animals with a humanized FcRn α-chain (FcRn-/- hFcRn line 276 Tg). Homozygous NSG FcRn-/- hFcRn (276) Tg mice have no mature T cells or B cells, lack functional natural killer (NK) cells, are deficient in cytokine signaling, and express human FcRn in place of the endogenous mouse FcRn. These mice may be useful in evaluating the pharmacokinetics/pharmacodynamics of human immunoglobulin G in transplantation studies, human tumor xenografts and mouse tumor allografts. For example, examining the ability of anti-FcRn to inhibit angiogenesis and other tumor/stroma interactions.

In addition to these NSG FcRn-/- hFcRn (276) Tg mice (Stock No. 028616), the NSG FcRn-/- animals are also available with the hFcRn (32) Tg as Stock No. 028615.

Donating Investigator

Dr. Derry Roopenian, The Jackson Laboratory

Dr. Leonard D. Shultz, The Jackson Laboratory

Genetic overview

Genetic Background	Generation

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Il2rg^tm1Wjl

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Il2rg	interleukin 2 receptor, gamma chain

Fcgrt^tm1Dcr

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Fcgrt	Fc receptor, IgG, alpha chain transporter

Tg(CAG-FCGRT)276Dcr

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Virology Research
Cancer Research
Research Tools
Internal/Organ Research
Cardiovascular Research
Hematological Research
Developmental Biology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

NSG FcRn-/- hFcRn (276) Tg mice are NOD.scid.Il2Rγc^null ("NSG") animals with a humanized FcRn α-chain (FcRn-/- hFcRn line 276 Tg).

NSG FcRn-/- hFcRn (276) Tg females homozygous for all three mutations and the transgene, and males homozygous for scid, homozygous for FcRn null, hemizygous for the X-linked Il2Rγc^null and homozygous for the hFcRn(276) transgene may be collectively referred to as homozygous NSG FcRn-/- hFcRn (276) Tg mice.

Although viable and fertile, homozygous NSG FcRn-/- hFcRn (276) Tg mice are immunodeficient: they have no mature T cells or B cells, lack functional natural killer (NK) cells and are deficient in cytokine signaling. They have endogenous FcRn α-chain expression deleted and replaced a human FcRn α-chain (FCGRT) transgene under control of CAG hybrid promoter.

For additional information on the phenotype of FcRn-/- hFcRn (276) Tg mice, see Stock No. 004919.

Similar to other immunodeficient strains, maintaining homozygous NSG FcRn-/- hFcRn (276) Tg mice in high health status (specific pathogen-free) vivaria promotes overall colony health. If homozygous NSG FcRn-/- hFcRn (276) Tg animals are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.

View our
Resources for the NSG mouse model, including discussion forum, immunodeficient model comparison, and categorized, up-to-date references.

Development

NSG FcRn-/- hFcRn (276) Tg mice are NOD.scid.Il2Rγc^null ("NSG") animals with the FcRn knock-out allele and the hFcRn (276) transgene expressing human FcRn α-chain. Each mutation/transgene are described below.

NSG mice are non-obese diabetic animals (NOD/ShiLtJ) harboring the spontaneous severe combined immunodeficiency mutation (Prkdc^scid) on chromosome 16 and the Il2Rγc^null mutation at ~101 Mbp on the X chromosome (Il2rg^tm1Wjl created by Dr. Warren J. Leonard [NIH]). NSG mice are described and available from The Jackson Laboratory Repository as Stock No. 005557.

The FcRn α-chain knock-out allele (Fcgrt^tm1Dcr) was generated in the laboratory of Dr. Derry Roopenian (The Jackson Laboratory), and is described for Stock No. 003982. Briefly, it has 1588 nucleotides (encoding promoter sequence 5' of the transcriptional start site, exon1, intron 2, and most of exon2) replaced with a PGK-Neo^r cassette.

The hFcRn (276) transgene (Tg(CAG-FCGRT)276Dcr) was generated in the laboratory of Dr. Derry Roopenian (The Jackson Laboratory), and is described for Stock No. 004919. Briefly, the transgene was designed with the 0.34 kb human cytomegalovirus immediate early promoter enhancer, 1.4 kb chicken beta-actin promoter and intron 1, a cDNA sequence encoding the human FcRn α-chain (FCGRT), 0.73 kb rabbit beta-globin intron, and 0.35 kb SV40 polyA sequence.

In 2016, some C57BL/6J-congenic FcRn-/- hFcRn (276) Tg mice (from Stock No. 004919) were bred to NSG mice (from Stock No. 005557) for five generations. The resulting NSG FcRn-/- hFcRn (276) Tg mice were bred together for several generations, and then made available as Stock No. 028616. In November 2017, SNP analyses show the colony to be greater than 97% NOD.

Expression Data

Expressed Gene	FCGRT, Fc fragment of IgG receptor and transporter, human
Site of Expression

Control Suggestions

Depending upon the experiment, the following may be appropriate control strain(s):
--NOD.scid.IL2RγcKO ("NSG"; Stock No. 005557)
--NOD.scid (Stock No. 001303)
--NOD/ShiLtJ inbred mice (Stock No. 001976)
--FcRn-/- hFcRn (276) Tg (Stock No. 004919)
--FcRn-/- on NOD (Stock No. 003988)

Approximate Controls

Additional Information

Considerations for Choosing Controls

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Allele Name	targeted mutation 1, Warren J Leonard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	[KO]gammac; CD132^-; gammac^-; gc^-; Il2rg^tm1Wjll; IL2Rgamma^null
Gene Symbol and Name	Il2rg, interleukin 2 receptor, gamma chain
Gene Synonym(s)
Site of Expression	Primarily lymphoid cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.
Mutations Made By	Dr. Warren Leonard, NHLBI, NIH

Fcgrt^tm1Dcr

Allele Symbol: Fcgrt^tm1Dcr

Allele Name	targeted mutation 1, Derry C Roopenian
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	FcRn^-
Gene Symbol and Name	Fcgrt, Fc receptor, IgG, alpha chain transporter
Gene Synonym(s)
Strain of Origin	129X1/SvJ
Chromosome	7
Molecular Note	Sequence from exon 1 and part of exon2 was replaced with a PGK-neo cassette. Quantitative PCR of liver cDNA indicated the absence of mRNA. Western blot analysis of neonatal intestinal extracts failed to reveal protein product.

Tg(CAG-FCGRT)276Dcr

Allele Symbol: Tg(CAG-FCGRT)276Dcr

Allele Name	transgene insertion 276, Derry C Roopenian
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	hFcRn (276) Tg; Tg276
Gene Symbol and Name	Tg(CAG-FCGRT)276Dcr, transgene insertion 276, Derry C Roopenian
Gene Synonym(s)
Promoter	ACTB, actin, beta, chicken
Expressed Gene	FCGRT, Fc fragment of IgG receptor and transporter, human
Strain of Origin	C57BL/6J
Chromosome	1
Molecular Note	To generate the hFcRn line 276 transgenic mice, a transgene was designed with the 0.34 kb human cytomegalovirus immediate early promoter enhancer, 1.4 kb chicken beta-actin promoter and intron 1, a cDNA sequence encoding the human FcRn alpha-chain, 0.73 kb rabbit beta-globin intron, and 0.35 kb SV40 polyA sequence. Line 276 was established. Transgene insertion occurred on Chr 1, causing a 1 bp duplication.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdc^scid related

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool
Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency

Genotype: Fcgrt^tm1Dcr related

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - defects in humoral immune responses
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin
- Autoimmunity
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - genes regulating susceptibility to infectious disease and endotoxin

Genotype: Il2rg^tm1Wjl related

Cancer Research
- Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Research Tools
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
  - T cell deficiency
  - production of B and T cells, antibodies, and hybridomas
  - genes regulating lymphoma development
  - xenograft/transplant host
  - B, T, and NK cell deficiency, xenograft/transplant host
  - production of T cells and hybridoma
  - B cell deficiency
  - production of B cells and antibodies
- Immunology, Inflammation and Autoimmunity Research
  - B and T cell deficiency
  - specific complement deficiency
  - Macrophage Deficiency
  - T cell deficiency
  - B, T, and NK cell deficiency
  - NK Cell Deficiency
  - MHC class II defects
  - background strain for histocompatibility congenics
  - genes regulating susceptibility to infectious disease and endotoxin
  - T cell deficiency, xenograft/transplant host
  - production of T cell lines and hybridomas
  - B cell deficiency
  - production of B cells and antibodies
  - production of B cells, antibodies T cell lines, and hybridomas
- Toxicology Research
  - xenograft/transplant host
  - B and T cell deficiency, xenograft transplant host
Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin
- Autoimmunity
  - specific complement deficiency
  - B and T cell deficiency
  - B cell deficiency
- Immunodeficiency
  - specific complement deficiency
  - multiple immune defects
  - Macrophage defects
  - B and T cell deficiency
  - T cell deficiency
  - NK Cell Deficiency
  - MHC class II defects
  - MHC class II deficient
  - NK Cell and Cd8 T Cell defects
  - defects in humoral immune responses
  - B cell defects
  - B cell deficiency
  - B, T, and NK cell deficiency
- T Cell Receptor Signaling Defects
  - B and T cell deficiency, xenograft/transplant host
  - B and T cell deficiency
- Immunodeficiency Associated with Other Defects
- HLA transgenics
- Lymphoid Tissue Defects
  - B and T cell deficiency
  - hematopoietic development
- Graft vs. Host Disease
- Inflammation
  - B and T cell deficiency
- Growth Factors/Receptors/Cytokines
Internal/Organ Research
- Lymphoid Tissue Defects
  - T cell deficiency
  - B and T cell deficiency
- Thymus Defects
  - B and T cell deficient
Virology Research
- B and T Cell Deficiency
  - AIDS research tool
Cardiovascular Research
- Vascular Defects
  - defective macrophage function
Cancer Research
- Toxicology
  - B and T cell deficiency, xenograft/transplant host
  - xenograft/transplant host
- Growth Factors/Receptors/Cytokines
Hematological Research
- Immunological Defects
  - B and T cell deficiency
- Hematopoietic Defects
Developmental Biology Research
- Lymphoid Tissue Defects
  - hematopoietic defects
- Internal/Organ Defects
  - hematopoietic defects
  - Lymphoid Tissue Defects

Mammalian Phenotype Terms by Genotype

References

Additional - Fcgrt^tm1Dcr related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related

Additional - Tg(CAG-FCGRT)276Dcr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Tg(FCGRT) alternate1
- Probe:Il2rg-PROBE
- End Point Analysis:Prkdc End Point
- Standard PCR:Fcgrt Alternate1
- Genotyping resources and troubleshooting
Breeding Considerations

NSG FcRn-/- hFcRn (276) Tg females homozygous for all three mutations and the transgene, and males homozygous for scid, homozygous for FcRn null, hemizygous for the X-linked Il2Rγc^null and homozygous for the hFcRn(276) transgene may be collectively referred to as homozygous NSG FcRn-/- hFcRn (276) Tg mice. When maintaining a live colony, homozygous NSG FcRn-/- hFcRn (276) Tg females may be bred with homozygous NSG FcRn-/- hFcRn (276) Tg males.

Homozygous NSG FcRn-/- hFcRn (276) Tg mice are immunodeficient. As such, and similar to other immunodeficient strains, maintenance in high health status (specific pathogen-free) vivaria promotes overall colony health. If homozygous NSG FcRn-/- hFcRn (276) Tg animals are maintained in low health barrier rooms, the use of medicated water (e.g., sulfatrim/trimethoprim-sulfa or enrofloxacin/Baytril) is suggested to increase overall colony health.
- Additional Breeding and Husbandry Support
Citation
When using the NSG FcRn-/- hFcRn (276) Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #028616 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Domestic Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

International Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Fcgrt<tm1Dcr> Heterozygous for Prkdc<scid> Heterozygous for Il2rg<tm1Wjl> Hemizygous for Tg(CAG-FCGRT) females orHeterozygous for Fcgrt<tm1Dcr> Heterozygous for Prkdc<scid> Wildtype for Il2rg<tm1Wjl> Hemizygous for Tg(CAG-FCGRT) males

Related Products and Services

Frozen Mouse Embryo	NOD.Cg-Tg(CAG-FCGRT)276Dcr Fcgrt<tm1Dcr> Prkdc<scid> Il2rg<t	$2595.00
Frozen Mouse Embryo	NOD.Cg-Tg(CAG-FCGRT)276Dcr Fcgrt<tm1Dcr> Prkdc<scid> Il2rg<t	$2595.00
Frozen Mouse Embryo	NOD.Cg-Tg(CAG-FCGRT)276Dcr Fcgrt<tm1Dcr> Prkdc<scid> Il2rg<t	$3373.50
Frozen Mouse Embryo	NOD.Cg-Tg(CAG-FCGRT)276Dcr Fcgrt<tm1Dcr> Prkdc<scid> Il2rg<t	$3373.50

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Use of MICE by companies or for-profit entities requires a no-fee JAX Leap License prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NSG FcRn-/- hFcRn (276) Tg

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Prkdcscid

Allele Symbol: Prkdcscid

Il2rgtm1Wjl

Allele Symbol: Il2rgtm1Wjl

Fcgrttm1Dcr

Allele Symbol: Fcgrttm1Dcr

Tg(CAG-FCGRT)276Dcr

Allele Symbol: Tg(CAG-FCGRT)276Dcr

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdcscid related

Genotype: Fcgrttm1Dcr related

Genotype: Il2rgtm1Wjl related

Mammalian Phenotype Terms by Genotype

References

Additional - Fcgrttm1Dcr related

Additional - Il2rgtm1Wjl related

Additional - Prkdcscid related

Additional - Tg(CAG-FCGRT)276Dcr related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Prkdc^scid

Allele Symbol: Prkdc^scid

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Fcgrt^tm1Dcr

Allele Symbol: Fcgrt^tm1Dcr

Genotype: Prkdc^scid related

Genotype: Fcgrt^tm1Dcr related

Genotype: Il2rg^tm1Wjl related

Additional - Fcgrt^tm1Dcr related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related